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ALX4 Antibody (KABB4)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
KABB4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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ALX4 Antibody (KABB4) Summary

Immunogen
Recombinant full length protein (Human).
Localization
Nuclear
Specificity
ALX4 (KABB4)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALX4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
Application Notes
Detects bands of approximately 41 and 43 kDa.
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
0.08% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for ALX4 Antibody (KABB4)

  • ALX homeobox 4
  • FND2
  • homeobox protein aristaless-like 4
  • homeodomain transcription factor ALX4
  • KIAA1788

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: MSX2 Antibody [NBP1-85445] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: MSX2 Antibody [NBP1-85445] - Staining of human urinary bladder shows weak nuclear positivity in urothelial cells.
Immunohistochemistry-Paraffin: MSX2 Antibody [NBP1-85445] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
(3 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
Sonic Hedgehog/Shh
[Unconjugated]
1845-SH
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029  is a matrix-associated artifact of the MALDI-TOF.
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 μg/mL.
(5 Reviews)
(36 Publications)
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CART1 Antibody
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Western Blot: CART1 Antibody [NBP1-88189] - Analysis in control (vector only transfected HEK293T lysate) and ALX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: CART1 Antibody [NBP1-88189] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & the Golgi apparatus.
Immunohistochemistry-Paraffin: CART1 Antibody [NBP1-88189] - Staining of human cervix, uterine shows moderate nuclear positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Recombinant Human
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H00000257-Q01
SDS-Page: Recombinant Human ALX3 Protein [H00000257-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
GLI-3 Antibody
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Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI‑3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI‑3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The gli-1 promoter was detected by standard PCR.
GLI‑3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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(35 Publications)
Species: Hu, Mu
Applications: WB, ChIP, ICC
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Western Blot: Ext2 Antibody [NBP1-58297] - Human Hela, Antibody Dilution: 1.0 ug/ml EXT2 is supported by BioGPS gene expression data to be expressed in HeLa.
Immunohistochemistry: Ext2 Antibody [NBP1-58297] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
Western Blot: Ext2 Antibody [NBP1-58297] - 721_B cell lysate, concentration 0.2-1 ug/ml.
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Applications: WB, IHC, IHC-P
Septin-9 Antibody
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(10E4E6)
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Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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[Unconjugated]
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Western Blot: TMEFF2 Antibody (1F9) [NBP2-01863] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEFF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEFF2.
Immunohistochemistry-Paraffin: TMEFF2 Antibody (1F9) [NBP2-01863] Staining of paraffin-embedded Human tonsil using anti-TMEFF2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TMEFF2 Antibody (1F9) [NBP2-01863] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TMEFF2 mouse monoclonal antibody.
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FGFR1 Antibody
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Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) Positive cells, (Green) Negative cells, (Blue) Negative cells + antibody, (Pink) Positive cells + antibody. Image from verified customer review.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (Dark Blue) and Isotype control (Orange). An antibody concentration of 1 ug/1x10^6 cells was used.
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FGF-8
[Unconjugated]
423-F8/CF
1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8/CF) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.
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SS18L1 Antibody
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Western Blot: SS18L1 Antibody [NBP2-20486] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: SS18L1 Antibody [NBP2-20486] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SS18L1 Antibody [NBP2-20486] - Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using antibody at 1:500 dilution.
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Applications: WB, ICC/IF, IHC, IHC-P, IP
ALX4 Antibody
(KABB4)
NB120-11827
ALX4 Antibody (KABB4)
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol ALX4
Entrez
Uniprot