ALX3 Antibody

Images

 
Western Blot: ALX3 Antibody [NB100-92297] - Analysis in extracts from 293 cells.
Immunohistochemistry-Paraffin: ALX3 Antibody [NB100-92297] - Paraffin-embedded human kidney carcinoma tissue at 1:50,showing nucleus staining. Negative control (the right) Using PBS instead of primary antibody, ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92297
    • Availability
      Product Discontinued

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ALX3 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 171-220 of Human ALX3.
Specificity
ALX3 (R203)Antibody detects endogenous levels of ALX3 (R203) protein.
Clonality
Polyclonal
Host
Rabbit
Gene
ALX3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of ALX3 (R203) pAb in extracts from 293 cells.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ALX3 Antibody

  • ALX homeobox 3
  • aristaless-like homeobox 3
  • homeobox protein aristaless-like 3
  • MGC138212
  • MGC141988
  • Proline-rich transcription factor ALX3

Background

Aristaless-related genes are a group of paired-related homeobox genes which play a role in regulating vertebrate embryogenesis. The homeodomain transciption factor aristaless-like 3 (ALX3) is expressed in mouse embryos from 8 days of gestation, predominantly in neural crest-derived mesenchyme and in lateral plate mesoderm. Expression analysis of human and mouse tissue reveals predominant ALX3 expression in brain tissue. The Alx3 gene maps to chromosome 1p23-p13 and encodes a 343 amino acid protein. Preferential methylation of Alx3 occurs in advanced-stage neuroblastoma and may repress ALX3 expression. Treatment with the methylation inhibitor 5-aza-2'-deoxycytidine restores ALX3 expression. Alx3 (-) mice lack a phenotype distinct from wild-type mice, however Alx3/Alx4 double mutants demonstrate severe craniofacial abnormalities not present in Alx4 single mutants. Specifically, Alx3/Alx4 double mutant newborn mice have cleft nasal regions in addition to malformation of other neural crest-derived skull structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALX3
Entrez