AlphaB Crystallin/CRYAB Antibody (3C2)

Images

 
Western Blot: Crystallin AB Antibody (3C2) [NBP1-47712] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Crystallin AB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunohistochemistry-Paraffin: Crystallin AB Antibody (3C2) [NBP1-47712] - Staining of paraffin-embedded Human lymphoma tissue using anti-Crystallin AB mouse monoclonal antibody.
Flow Cytometry: Crystallin AB Antibody (3C2) [NBP1-47712] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Crystallin AB antibody, ...read more
Immunohistochemistry-Paraffin: Crystallin AB Antibody (3C2) [NBP1-47712] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Crystallin AB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Crystallin AB Antibody (3C2) [NBP1-47712] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Crystallin AB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Crystallin AB Antibody (3C2) [NBP1-47712] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Crystallin AB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Crystallin AB Antibody (3C2) [NBP1-47712] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Crystallin AB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Crystallin AB Antibody (3C2) [NBP1-47712] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Crystallin AB mouse monoclonal antibody.
Immunoprecipitation: Crystallin AB Antibody (3C2) [NBP1-47712] - Lane 1: Negative Control Lane 2: NBP1-47852 Lane 3: NBP1-47712 Lane 4: NBP1-47707 Lane 5: NBP1-47708 Lane 6: NBP1-47709 Lane 7: NBP1-47710 Lane 8: ...read more

Product Details

Summary
Product Discontinued
View other related AlphaB Crystallin/CRYAB Primary Antibodies

Order Details


    • Catalog Number
      NBP1-47712
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

AlphaB Crystallin/CRYAB Antibody (3C2) Summary

Immunogen
Protein expressed in 293T cell transfected with human CRYAB expression vector
Specificity
This antibody is specific for Homo sapiens crystallin, alpha B (CRYAB).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CRYAB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody (3C2)

  • alpha B crystallin
  • alpha(B)-crystallin
  • AlphaB Crystallin
  • alpha-crystallin B chain
  • CRYA2
  • CRYA2alpha crystallin B chain
  • CRYAB
  • crystallin, alpha B
  • CTPP2
  • Heat shock protein beta-5
  • heat-shock 20 kD like-protein
  • HSPB5
  • Renal carcinoma antigen NY-REN-27
  • Rosenthal fiber component

Background

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development,these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-32972
Species: Hu, Mu, Rt, Ch, Pm, Sh
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-46354
Species: Hu
Applications: WB, IHC, IF
NB120-15200
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-02576
Species: Hu, Rt, Mk
Applications: WB, Flow, ICC/IF
NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-02459
Species: Hu, Mu, Rt, Ch, Dr, Pm
Applications: WB, ELISA, IHC, IHC-P
MAB4200
Species: Hu, Mu, Rt
Applications: WB
NBP1-81696
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
AF2584
Species: Mu
Applications: WB, Simple Western
31310002
Species: Hu
Applications: WB, ELISA
H00001415-M02
Species: Hu
Applications: WB, ELISA
NBP1-32557
Species: Hu
Applications: WB
NBP1-87851
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-26380
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-47712
Species: Hu
Applications: WB, Flow, IHC, IHC-P, IP

Publications for AlphaB Crystallin/CRYAB Antibody (NBP1-47712) (0)

There are no publications for AlphaB Crystallin/CRYAB Antibody (NBP1-47712).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AlphaB Crystallin/CRYAB Antibody (NBP1-47712) (0)

There are no reviews for AlphaB Crystallin/CRYAB Antibody (NBP1-47712). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AlphaB Crystallin/CRYAB Antibody (NBP1-47712) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional AlphaB Crystallin/CRYAB Products

Bioinformatics Tool for AlphaB Crystallin/CRYAB Antibody (NBP1-47712)

Discover related pathways, diseases and genes to AlphaB Crystallin/CRYAB Antibody (NBP1-47712). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AlphaB Crystallin/CRYAB Antibody (NBP1-47712)

Discover more about diseases related to AlphaB Crystallin/CRYAB Antibody (NBP1-47712).
 

Pathways for AlphaB Crystallin/CRYAB Antibody (NBP1-47712)

View related products by pathway.

PTMs for AlphaB Crystallin/CRYAB Antibody (NBP1-47712)

Learn more about PTMs related to AlphaB Crystallin/CRYAB Antibody (NBP1-47712).
 

Research Areas for AlphaB Crystallin/CRYAB Antibody (NBP1-47712)

Find related products by research area.

Blogs on AlphaB Crystallin/CRYAB

There are no specific blogs for AlphaB Crystallin/CRYAB, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AlphaB Crystallin/CRYAB Antibody (3C2) and receive a gift card or discount.

Bioinformatics

Gene Symbol CRYAB
Entrez