alpha Tubulin Antibody

Images

 
Western Blot: alpha Tubulin Antibody [NB100-92249] - Analysis of Tubulin alpha (G436) pAb at 1:5000/1:10000/1:20000 dilutionLane1:HEK293T whole cell lysate(40ug)Lane2:Hela whole cell lysate(40ug)Lane3:AML-12 whole cell ...read more
Immunohistochemistry: alpha Tubulin Antibody [NB100-92249] - Analysis of paraffin embedded breast carcinoma tissue. Used at a dilutino of 1:100
Western Blot: alpha Tubulin Antibody [NB100-92249] - Extracts from 293 cells.
Western Blot: alpha Tubulin Antibody [NB100-92249] - Analysis of 3 lysates. Lane 1: MCF-7 Lane 2: Mouse brain tissue Lane 3: Rat brain tissue
Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NB100-92249] - Paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92249
    • Availability
      Product Discontinued

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alpha Tubulin Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 201-256 of Human Tubulin alpha.
Marker
Microtubule Marker
Specificity
Alpha tubulin antibody detects tubulin alpha protein.
Clonality
Polyclonal
Host
Rabbit
Gene
TUBA1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Tubulin alpha (G436) pAb in extracts from 293 cells.
Immunohistochemistry (IHC) analyzes of Tubulin alpha (G436) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
alpha Tubulin Lysate (NBL1-17427)
Publications
Read Publication using NB100-92249.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha Tubulin Antibody

  • alpha Tubulin
  • Alpha-Tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA1A
  • TUBA3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-Alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha Tubulin Antibody (NB100-92249)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin Antibody (NB100-92249). (Showing 1 - 6 of 6 FAQ).

  1. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.
  2. We are working with mouse liver tissue, will this alpha tubulin antibody work as a loading control?
    • Yes, alpha tubulin is expressed in nearly all tissue types including liver; however we do not recommend using as a loading control antibody in stomach or oral mucosa tissue as expression of alpha tubulin in these areas is minimal.
  3. Will your alpha tubulin antibody work as a loading control for simple western?
    • Yes, this alpha tubulin antibody has been validated in simple western and makes an excelent loading control antibody. Simple western has a lower detection limit of 12kDA and alpha tubulin detects around 55kDA.
  4. Will this alpha tubulin antibody recognize both isoforms of alpha tubulin?
    • The epitope for this alpha tubulin antiboyd lies on the C-terminus of the protein and the difference between the two major isoforms is witin the first 35 aa of the N-terminus so this alpha tubulin antibody will recognize both isoforms.
  5. We're looking at LC3B, aSYN and p62 which are 18, 23 and 65 kDA respectively so we need the loading control antibody to be between those.
    • Alpha tubulin antibody detects around 55kDA so that would be a good option for a loading control antibody for those targets as you should be able to easily distinguish from the other bands.
  6. I am looking for a primary alpha tubulin  antibody that is conjugated to a red fluorophore.
    • Okay, our most popular alpha tubulin antibody is NB100-690 and is available in a variety of formats. So if you are looking for red I would recommend a DyLight 550, 650 etc.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on alpha Tubulin.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Alpha Tubulin: A Fundamental Cytoskeleton Protein with Many Roles
The cytoskeleton is consists of three major type of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Tubulins are the microtubule building block and exist as globular dimeric proteins of alpha/beta chains....  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

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Bioinformatics

Gene Symbol TUBA1A
Entrez
Uniprot