alpha-Smooth Muscle Actin Antibody (SP171)

Images

 
Western Blot: Actin Alpha 2 Smooth Muscle Antibody (SP171) [NBP2-21748] - HeLa cell lysate
Immunohistochemistry-Paraffin: Actin Alpha 2 Smooth Muscle Antibody (SP171) [NBP2-21748] - Human colon.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
SP171
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

alpha-Smooth Muscle Actin Antibody (SP171) Summary

Immunogen
Synthetic peptide near the N-terminus of human SMA protein.
Localization
Cytoplasm
Marker
Mesenchymal Cell Marker
Clonality
Monoclonal
Host
Rabbit
Gene
ACTA2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
Expect a band at ~42 kDa by western blot.

Reactivity Notes

Tested in Human. Based on sequence identity cross reactivity is expected with Bovine, Chicken, Mouse, Porcine, Rabbit and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Purity
Protein G purified

Alternate Names for alpha-Smooth Muscle Actin Antibody (SP171)

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin

Background

Smooth muscle actin-alpha (SMA) is a cytoskeleton protein in smooth muscle cells and their derived tumors such as leiomyoma and leiomyosarcoma. It is also expressed in myoepithelial cells of the breast and salivary gland, but not in fibroblasts, striated muscle, and myocardium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for alpha-Smooth Muscle Actin Antibody (NBP2-21748)

Discover related pathways, diseases and genes to alpha-Smooth Muscle Actin Antibody (NBP2-21748). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on alpha-Smooth Muscle Actin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

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Bioinformatics

Gene Symbol ACTA2
Entrez