alpha-Galactosidase A/GLA Recombinant Protein Summary
A recombinant protein with a His-tag corresponding to amino acids 1 to 451 of E.coli alpha-Galactosidase A/GLA
Source: E. coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMSAPKI TFIGAGSTIF VKNILGDVFH REALKTAHIA LMDIDPTRLE ESHIVVRKLM DSAGASGKIT CHTQQKEALE DADFVVVAFQ IGGYEPCTVT DFEVCKRHGL EQTIADTLGP GGIMRALRTI PHLWQICEDM TEVCPDATML NYVNPMAMNT WAMYARYPHI KQVGLCHSVQ GTAEELARDL NIDPATLRYR CAGINHMAFY LELERKTADG SYVNLYPELL AAYEAGQAPK PNIHGNTRCQ NIVRYEMFKK LGYFVTESSE HFAEYTPWFI KPGREDLIER YKVPLDEYPK RCVEQLANWH KELEEYKKAS RIDIKPSREY ASTIMNAIWT GEPSVIYGNV RNDGLIDNLP QGCCVEVACL VDANGIQPTK VGTLPSHLAA LMQTNINVQT LLTEAILTEN RDRVYHAAMM DPHTAAVLGI DEIYALVDDL IAAHGDWLPG WLHR
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 53 kDa (474aa)
Alternate Names for alpha-Galactosidase A/GLA Recombinant Protein
- agalsidase alfa
- Agalsidase alpha
- Alpha-D-galactosidase A
- alpha-D-galactoside galactohydrolase 1
- Alpha-D-galactoside galactohydrolase
- alpha-gal A
- alpha-galactosidase A
- EC 3.2.1
- EC 22.214.171.124
- galactosidase, alpha
melA(alpha-galactosidase) belongs to glycosyl hydrolase 4 family. Alpha-galactosidases catalyse the hydrolysis of saccharides containing o-1,6,-galactoside linkages. The three alpha-galactosidases catalyse the same reaction, but are localized in different cellular compartments: The E.coli enzyme is cytoplasmic protein and the human enzyme and the yeast enzyme is secretory proteins. Therefore, although the active enzyme from all three species has nearly the same molecular weight, structural similarities, as well as differences, are expected. Recombinant E.coil melA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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