alpha Adducin Antibody

Images

 
Western Blot: alpha Adducin Antibody [NBP1-48611] - Analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1 mM, 30 min) using Rabbit Anti-ADD1 (Ab-726) Polyclonal Antibody (GenScript, A00925, lane ...read more
Immunocytochemistry/ Immunofluorescence: alpha Adducin Antibody [NBP1-48611] - Staining of methanol-fixed Hela cells using.
Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP1-48611] - Analysis of paraffin-embedded human lung carcinoma tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

alpha Adducin Antibody Summary

Immunogen
Synthesized non-phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-S P -F-L)
Specificity
Rabbit Anti-ADD1 (Ab-726) Polyclonal Antibody detects endogenous levels of total ADD1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADD1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100

Reactivity Notes

Human, mouse, rat

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg 2+ and Ca 2+), pH 7.4, 150mM NaCl, and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha Adducin Antibody

  • ADDA
  • adducin 1 (alpha)
  • alpha-adducin
  • erythrocyte adducin alpha subunit
  • Erythrocyte adducin subunit alpha
  • MGC3339
  • MGC44427

Background

Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; alpha and beta, or alpha and gamma subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser59, Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ADD1
Uniprot