Alpha Actinin (pan) Antibody

Product Discontinued

Alpha Actinin (pan) Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 30-70 of Human Actinin-alpha2.
• Specificity: Recognizes both alpha-actinin-2 and alpha-actinin-3. Alpha-actinin-2 (Gene ID: 59, UniProt: P62736); Alpha-actinin-3 (Gene ID: 89, UniProt: Q08043).
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Alpha Actinin (pan) Antibody

• actinin 1 smooth muscle
• actinin, alpha 1
• alpha-actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins,including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple rolesin different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles andadherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, andsmooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillaractin filaments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN1