Alpha Actinin 3 Antibody


Western Blot: Alpha Actinin 3 Antibody [NB100-92434] - Extracts from HT-29 cells.
Western Blot: Alpha Actinin 3 Antibody [NB100-92434] - Extracts from PC12 cells.

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Alpha Actinin 3 Antibody Summary

Synthetic peptide, corresponding to the N-terminus of Human Actinin-alpha3.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
15mM Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Alpha Actinin 3 Antibody

  • actinin, alpha 3
  • Alpha-actinin skeletal muscle isoform 3
  • alpha-actinin skeletal muscle
  • alpha-actinin-3
  • F-actin cross-linking protein
  • MGC117002
  • MGC117005


Focal adhesions were identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex, which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and alpha-actinin. In addition, vinculin, Talin and alpha-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. alpha-actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and talin complex or by a direct interaction with integrin.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol ACTN3