alpha-Actinin 1 Antibody (EP2528Y)

Product Discontinued

alpha-Actinin 1 Antibody (EP2528Y) Summary

• Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human Alpha-Actinin 1 was used as an immunogen.
• Marker: Z-disk Marker
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACTN1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:40
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunoprecipitation 1:40
  • Western Blot 1:500 - 1000

Reactivity Notes

Reacts with: Mouse, Rat, Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for alpha-Actinin 1 Antibody (EP2528Y)

• actinin 1 smooth muscle
• actinin, alpha 1
• ACTN1
• alpha Actinin 1
• alphaActinin 1
• alpha-Actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• BDPLT15
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoforms are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN1
• Entrez:
  • 87(Human)
• Uniprot:
  • P12814()