alpha-Actinin 1 Antibody (7A4)

Images

 
Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Alpha Actinin 1 antibody (7A4) at 1:2000 + recombinant human Alpha Actinin 1.
Immunocytochemistry/ Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
7A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

alpha-Actinin 1 Antibody (7A4) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 650-893 of human alpha-actinin
Marker
Z-disk Marker
Specificity
This antibody is specific for Homo sapiens actinin, alpha 1 (ACTN1), transcript variant 2.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ACTN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Publications
Read Publications using
NBP1-48251 in the following applications:

  • IHC
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27512039).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for alpha-Actinin 1 Antibody (7A4)

  • actinin 1 smooth muscle
  • actinin, alpha 1
  • ACTN1
  • alphaActinin 1
  • alpha-Actinin 1
  • Alpha-actinin cytoskeletal isoform
  • alpha-actinin-1
  • BDPLT15
  • F-actin cross-linking protein
  • FLJ40884
  • FLJ54432
  • Non-muscle alpha-actinin-1

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Actinin 1 Antibody (NBP1-48251)(2)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACTN1
Entrez