Alkaline Phosphatase, Tissue Non-Specific Antibody [HRP]

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Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody [HRP] [NB120-7319] - Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody ...read more

Order Details


    • Catalog Number
      NB120-7319
    • Availability
      Product Discontinued

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Alkaline Phosphatase, Tissue Non-Specific Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Full length protein (E. coli).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli].
Clonality
Polyclonal
Host
Rabbit
Gene
ALPL
Purity
Delipidation and Defibrination
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:20000
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
Readout System

Reactivity Notes

Cross-reacts with E. coli. Does not cross-react with all Mammals.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Purity
Delipidation and Defibrination

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody [HRP]

  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • APTNAP
  • AP-TNAP
  • EC 3.1.3.1
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALPMGC167935

Background

Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ALPL