ALK/CD246 Antibody (5A4)

Images

 
Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin-fixed, paraffin-embedded human anaplastic lymphoma stained with p80/ALK using peroxidase-conjugate and AEC chromogen. Note nuclear and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
5A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ALK/CD246 Antibody (5A4) Summary

Immunogen
Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus, corresponding to amino acids 419-520 of Human ALK.
Localization
Cell Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALK
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:25 - 1:70
Application Notes
IHC-P: An incubation period of 60 minutes at room temperature is recommendedc using an ABC method. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tissue culture supernatant
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for ALK/CD246 Antibody (5A4)

  • ALK tyrosine kinase receptor
  • ALK
  • anaplastic lymphoma kinase (Ki-1)
  • Anaplastic lymphoma kinase
  • anaplastic lymphoma receptor tyrosine kinase
  • CD246 antigen
  • CD246
  • EC 2.7.10.1
  • Ki-1
  • NBLST3
  • Tcrz

Background

Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkin's lymphomas. The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ALK
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