ALK/CD246 Antibody (5A4)

Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin fixed paraffin embedded anaplastic lymphoma kinase stained with ALK/CD246 antibody (NB120-17127).

Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin-fixed, paraffin-embedded human anaplastic lymphoma stained with p80/ALK using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of tumor cells.

• Reactivity: Hu
• Applications: IHC, IHC-P
• Clone: 5A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ALK/CD246 Antibody (5A4) Summary

• Immunogen: Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus, corresponding to amino acids 419-520 of Human ALK.
• Localization: Cell Membrane
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALK
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:25-1:70
  • Immunohistochemistry-Paraffin
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for ALK/CD246 Antibody (5A4)

• ALK tyrosine kinase receptor
• ALK
• anaplastic lymphoma kinase (Ki-1)
• Anaplastic lymphoma kinase
• anaplastic lymphoma receptor tyrosine kinase
• CD246 antigen
• CD246
• EC 2.7.10.1
• Ki-1
• NBLST3
• Tcrz

Background

Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkin's lymphomas. The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALK
• Entrez:
  • 238(Human)