Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein

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SDS-Page: AKR1C3 Protein [NBC1-21051] - AKR1C3, 39 kDa (343 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-323 of Human Aldo-keto Reductase 1C3/AKR1C3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY

Details of Functionality
Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
AKR1C3
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein

  • 17-beta-hydroxysteroid dehydrogenase type 5
  • 3-alpha-HSD type 2
  • 3-alpha-hydroxysteroid dehydrogenase type 2
  • AKR1C3
  • Aldo-keto Reductase 1C3
  • aldo-keto reductase family 1 member C3
  • aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
  • AldoketoReductase 1C3
  • Chlordecone reductase homolog HAKRb
  • DD3
  • DD-3
  • DD317-beta-HSD 5
  • DDH1
  • DDX
  • Dihydrodiol dehydrogenase 3
  • Dihydrodiol dehydrogenase type I
  • dihydrodiol dehydrogenase X
  • EC 1.1.1
  • EC 1.1.1.112,3-alpha-HSD type II, brain
  • EC 1.1.1.188
  • EC 1.1.1.213
  • EC 1.1.1.63
  • EC 1.1.1.64
  • EC 1.3.1.20
  • HA1753
  • HAKRB
  • HAKRBtype IIb 3-alpha hydroxysteroid dehydrogenase
  • HAKRe
  • HSD17B5
  • hydroxysteroid (17-beta) dehydrogenase 5
  • Indanol dehydrogenase
  • KIAA0119hluPGFS
  • PGFS
  • Prostaglandin F synthase
  • Testosterone 17-beta-dehydrogenase 5
  • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

Background

AKR1C3, also known as PGFS, is a member of the aldo-keto reductase superfamily which catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9 alpha, 11 beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and differentiation. Recombinant human AKR1C3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol AKR1C3
Entrez