alcohol dehydrogenase Antibody

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Western Blot: alcohol dehydrogenase Antibody [NB300-956] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: alcohol dehydrogenase Antibody [NB300-956] - Analysis of pan alcohol dehydrogenase in Human Liver lysate

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

alcohol dehydrogenase Antibody Summary

Immunogen
Peptide with sequence STAGKVMKCKA corresponding to N-Terminus according to NP_000658.1, NP_000659.2, NP_000660.1.
Specificity
This antibody is expected to recognise the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of human alcohol dehydrogenase.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADH1A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA detection limit 1:8000
Application Notes
WB: Approx. 38 kDa band observed in human liver lysates (calculated MW of 39.9 kDa band according to NP_000658.1, NP_000659.2 and 000660.1). IHC: An anonymous customer has reported on human liver.
Publications
Read Publication using NB300-956.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alcohol dehydrogenase Antibody

  • ADH, alpha subunit
  • ADH1alcohol dehydrogenase 1A
  • alcohol dehydrogenase 1 (class I), alpha polypeptide
  • alcohol dehydrogenase 1A (class I), alpha polypeptide
  • Alcohol dehydrogenase subunit alpha
  • aldehyde reductase
  • EC 1.1.1
  • EC 1.1.1.1

Background

This gene encodes class I alcohol dehydrogenase, alpha subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. This gene is monomorphic and predominant in fetal and infant livers, whereas the genes encoding beta and gamma subunits are polymorphic and strongly expressed in adult livers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ADH1A
Entrez
Uniprot