AKT2/3 Antibody

Images

 
Western Blot: AKT2/3 Antibody [NBP2-15330] - Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfect with AKT 7. 5% SDS PAGE; antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: AKT2/3 Antibody [NBP2-15330] - Paraformaldehyde-fixed HeLa, using AKT2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.65 mg/ml

Order Details

AKT2/3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Akt2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Zebrafish (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.65 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AKT2/3 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • Murine thymoma viral (v-akt) homolog-2
  • PKB beta
  • PKBB
  • PKBBETA
  • PRKBB
  • Protein kinase Akt-2
  • Protein kinase B beta
  • rac protein kinase beta
  • RAC-beta serine/threonine-protein kinase
  • RAC-BETA
  • RAC-PK-beta
  • v-akt murine thymoma viral oncogene homolog 2

Background

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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AKT2/3 NBP2-15330

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Bioinformatics

Gene Symbol AKT2
Uniprot