Akt1 [p Ser473, p Ser473, p Ser473, p Ser473] Antibody


Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody [NBP1-35280] - Human breast carcinoma.

Product Details

Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Akt1 [p Ser473, p Ser473, p Ser473, p Ser473] Antibody Summary

A synthetic peptide corresponding to the carboxyl terminus of AKT1/PKB alpha of human origin at the phosphorylated site of Serine 473.
p Ser473
This product is specific for AKT1/PKB (Ser 473)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
10mM PBS/1% BSA buffer pH 7.4
0.1% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


Application Notes
This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.

Reactivity Notes

This product is specific for Human.


For a positive control use Tonsil.

Alternate Names for Akt1 [p Ser473, p Ser473, p Ser473, p Ser473] Antibody

  • AKT
  • EC 2.7.11
  • EC
  • PKB alpha
  • PKBMGC99656
  • Protein kinase B
  • Proto-oncogene c-Akt
  • rac protein kinase alpha
  • RAC-alpha serine/threonine-protein kinase
  • RAC-alpha
  • RAC-PK-alpha
  • v-akt murine thymoma viral oncogene homolog 1


AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Akt1 Antibody (NBP1-35280). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.
    • At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.

Secondary Antibodies


Isotype Controls

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Blogs on Akt1.

AKT1 - Regulating cell growth and survival through phosphorylation
AKT1 is a serine/threonine protein kinase with homology to protein kinase A (PKA) and protein kinase C (PKC). AKT1 contains the central kinase domain sandwiched between a pleckstrin homology domain and a regulatory domain (1). AKT1 is regulated by...  Read full blog post.

Akt1 - a central player in cell survival signaling
Akt1 is one of three isoforms of Akt belonging to the AGC family of serine/threonine kinases (Akt1, Akt2, and Akt3). All Akt isoforms contain an N-terminal Plekstrin Homology (PH) domain, a C-terminal regulatory domain, and a central catalytic kin...  Read full blog post.

AKT1, Scene 1: The Cell Must Go On
Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis...  Read full blog post.

Caspase 9 and Mitochondrial Apoptosis Regulation
Caspase 9 (also termed ICE-LAP6, Mch6, Apaf-3) is a member of cysteine protease family of caspases and is encoded by the CASP9 gene in humans. Caspase-9 is involved in mitochondrial apoptosis pathway and is an initiator caspase. Pro-caspase-9 is activ...  Read full blog post.

Mapping Signal Transduction with mTOR Antibodies
The protein encoded by mTOR (mammalian target of rapamycin), also known as dTOR in Drosophila, belongs to a family of phosphatidylinositol kinase-related kinases. These kinases regulate fundamental processes of cell growth, proliferation, metabolism...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Antibodies Targeting the mTOR Pathway for Many Diseases
The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway that mediates cell survival and proliferation. It is increasingly apparent that mTOR signaling impac...  Read full blog post.

AKT Antibody Assays: A Complex Area with an Easy Solution
We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role...  Read full blog post.

The Akt Antibody Plays Many Roles in Cancer Research
There are three human isoforms of the AKT gene, which plays a key role in several signalling pathways. Akt antibody studies have shown the Atk kinases to play a diverse number of roles within the cell, regulating angiogenesis, apoptosis, protein synth...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol AKT1

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