Human, Mouse, Rat AKT [p Ser473, p Ser474] ELISA Kit (Colorimetric)

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ELISA: AKT ELISA Kit (Colorimetric) [NBP2-61397] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA
Suitable Sample Type
cell lysates of human, mouse, and rat origin.
Standard Curve Range
17.5 - 560 pg/ml
Sensitivity
5.5 pg/ml

Order Details

Human, Mouse, Rat AKT [p Ser473, p Ser474] ELISA Kit (Colorimetric) Summary

Modification
p Ser473, p Ser474
Specificity
This product has been tested with AKT1 - Ser473 and AKT2 - Ser474. Possible Cross Reactivity listed below: Inactive Akt1 0.05% Inactive Akt3 <0.03% p38a, inactive 0.17% ERK1, inactive <0.03% ERK2, inactive <0.03% JNK1, inactive <0.03%
Standard Curve Range
17.5 - 560 pg/ml
Sensitivity
5.5 pg/ml
Inter-Assay
Mean 9.7%
Intra-Assay
Mean 9.4%
Spike Recovery
Jurkat Cells - 97%
3T3 Cells - 90%
C6 Cells - 100%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AKT1

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Microtiter plate
  2. Conjugate
  3. Assay buffer 32
  4. Antibody
  5. Cell lysis buffer 5
  6. Standard
  7. Stop solution 2
  8. Wash buffer concentrate
  9. TMB Substrate

Alternate Names for Human, Mouse, Rat AKT [p Ser473, p Ser474] ELISA Kit (Colorimetric)

  • RAC-ALPHA
  • AKT serine/threonine kinase 1
  • Akt
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC

Background

Akt, also known as Protein Kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKBa), Akt2 (PKBB), and Akt3 (PKBY). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Blogs on AKT. Showing 1-10 of 26 blog posts - Show all blog posts.

Novel Insights into Hypoxia Induced AKT Signaling
Hypoxia is a common feature of most tumors and is a product of rapid cell growth and poor vascularization1. When oxygen availability is low in the tumor environment, the hypoxia inducing transcription factors (HIFs) regulate a variety of signaling...  Read full blog post.

Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis
Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to...  Read full blog post.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1,...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

Required proteins for p62/SQSTM1 regulation and a role for p62/SQSTM1 in neuronal autophagy
Autophagy is a crucial cellular process that clears the cell of protein aggregates, toxins, and damaged cell products. Accumulation of toxins, damaged cell products and unwanted proteins has been proven to play a role in aging and many forms of dis...  Read full blog post.

The role of LC3B and autophagy in alcohol induced liver disease
Autophagy is a crucial intracellular pathway that manages the degradation and recycling of long-lived proteins in the cell. The LC3 (or light chain 3) family is composed of three members, LC3A, LC3B and LC3C. Upon autophagy induction, LC3 is cleave...  Read full blog post.

Showing 1-10 of 26 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol AKT1