AKAP95/AKAP8 Antibody

Images

 
Western Blot: AKAP95/AKAP8 Antibody [NB100-1416] - NB100-1416 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AKAP95/AKAP8 Antibody Summary

Immunogen
Peptide with sequence DQGYGGYGAWSAG-C corresponding to N-Terminus according to NP_005849.1.
Localization
Nuclear. Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
Marker
Nucleus without Nucleoli Marker
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AKAP8
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Experiments gave a band at approx. 100 kDa band in human liver lysate after 1 ug/ml antibody staining. This band corresponds to the 95 kDa band reported in scientific literature (PMID: 10601332). This protein has a calculated MW of 76.1 kDa band according to NP_005849.
Positive Control
Liver Lysate (NB820-59232)
Publications
Read Publication using NB100-1416.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AKAP95/AKAP8 Antibody

  • A kinase (PRKA) anchor protein 8
  • AKAP-8
  • AKAP-95 AKAP 95
  • A-kinase anchor protein 8
  • A-kinase anchor protein 95 kDa
  • A-kinase anchor protein, 95kDa
  • DKFZp586B1222

Background

The A kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This antibody recognises a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKAP95/AKAP8 Antibody (NB100-1416)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for AKAP95/AKAP8 Antibody (NB100-1416)

Discover related pathways, diseases and genes to AKAP95/AKAP8 Antibody (NB100-1416). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on AKAP95/AKAP8.

AKAP95/AKAP8 Orchestrates and Synchronizes Cellular Events
A-kinase anchor proteins (AKAPs), such as AKAP95/AKAP8, are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA). AKAPs orchestrate and synchronize cellular events by tethering the cAMP-dependent PKA and oth...  Read full blog post.

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Bioinformatics

Gene Symbol AKAP8
Entrez
Uniprot