AKAP1 Antibody

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Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP1-19690] - Paraffin-embedded human brain tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-19690
    • Availability
      Product Discontinued

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AKAP1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 274-320 of Human AKAP 1.
Specificity
AKAP 149(N306) detects endogenous levels of AKAP 149 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
AKAP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of AKAP 149(N306) pAb in extracts from HUVEC cells.
Immunohistochemistry (IHC) analyzes of AKAP 149(N306) pAb in paraffin-embedded human brain tissue.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AKAP1 Antibody

  • A kinase (PRKA) anchor protein 1
  • a kinase anchor protein 1, mitochondrial
  • AKAP121
  • AKAP149AKAP 149
  • AKAP84
  • A-kinase anchor protein 1, mitochondrial
  • A-kinase anchor protein 149 kDa
  • A-kinase anchor protein, 149kD
  • D-AKAP1
  • D-AKAP-1
  • Dual specificity A-kinase-anchoring protein 1
  • dual-specificity A-kinase anchoring protein 1
  • MGC1807
  • PRKA1
  • protein kinase A anchoring protein 1
  • protein kinase A1
  • Protein kinase A-anchoring protein 1
  • protein kinase anchoring protein 1
  • S-AKAP84
  • SAKAP84AKAP
  • Spermatid A-kinase anchor protein 84

Background

The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. This family of proteins has been designated A-kinase anchoring proteins (AKAP). Members of this family, including MAP2 (microtubule-associated protein 2), neuronally expressed AKAP 79 and AKAP 150, and the DNA binding AKAP 95, display differential tissue specificity and localization. Evidence suggests that AKAP 79 and AKAP 150 are both capable of anchoring PKA to postsynaptic densities (PSD), which are a network of proteins located on the internal surfaces of excitatory synapses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol AKAP1
Entrez