AIF Antibody

Images

 
Western Blot: AIF Antibody [NBP1-77302] - AIF levels in human neuroblastoma. Image from verified customer review.
Immunohistochemistry-Paraffin: AIF Antibody [NBP1-77302] - Human retina with AIF antibody at 10 ug/ml.
Western Blot: AIF Antibody [NBP1-77302] - K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

AIF Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid peptide near the center of human AIF. The immunogen is located within amino acids 500 - 550 of AIF.
Specificity
At least five isoforms of AIF are known to exist; this antibody will detect all isoforms except isoform 5.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AIFM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
AIF antibody can be used for detection of AIF by Western blot at 0.25 to 1 mg/ml. A 67 kDa band should be detected.
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
AIF Peptide (NBP1-77302PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIF Antibody

  • AIF
  • AIFapoptosis-inducing factor 1, mitochondrial
  • AIFM1
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • COXPD6
  • MGC111425
  • PDCD8
  • PDCD8striatal apoptosis-inducing factor
  • programmed cell death 8 (apoptosis-inducing factor)
  • Programmed cell death protein 8

Background

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AIFM1
Entrez
Uniprot