Rat AGER ELISA Kit (Colorimetric)

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ELISA: AGER ELISA Kit [Biotin] [KA1835] - This standard curve is for demonstration only. A standard curve must be run with each assay.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum, Urine
Sensitivity
300 pg/mL

Order Details

Rat AGER ELISA Kit (Colorimetric) Summary

Description
Ager (Rat) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Ager.
Sensitivity
300 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AGER

Applications/Dilutions

Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
  2. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  3. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  4. Standards (Item C): 2 vials, recombinant rat RAGE.
  5. RAGE Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-rat RAGE.
  6. Assay Diluent D (Item K): 15 ml of 5x concentrated buffer. For Standard/Sample (serum/plasmasamples/cell culture medium/urine) diluent.
  7. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent.
  8. Detection Antibody RAGE (Item F): 2 vial of biotinylated anti-rat RAGE (each vial is enough to assay half microplate)
  9. HRP-Streptavidin concentrate (Item G): 8 µl 20,000x concentrated HRP-conjugated streptavidin.

Notes

Quality control test: Standard Curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rat AGER ELISA Kit (Colorimetric)

  • advanced glycosylation end product-specific receptor
  • MGC22357
  • RAGE isoform delta
  • RAGE isoform sRAGE-delta
  • RAGERAGE isoform NtRAGE-delta
  • Receptor for advanced glycosylation end products
  • receptor for advanced glycosylation end-products

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AGER