Aflatoxin B1 Antibody (5B3)

Product Discontinued

Aflatoxin B1 Antibody (5B3) Summary

• Immunogen: BSA-Aflatoxin
• Specificity: Reacts with aflatoxin B1
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000 - 1:10000

Reactivity Notes

Cross reacts with B2, G1 and G2

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with 0.2 ml distilled water.

Alternate Names for Aflatoxin B1 Antibody (5B3)

• AFB1
• AFB1-AR1
• Aldoketoreductase 7

Background

The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450-dependent) in the tissues (in particular, the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA and protein. Cytochrome P450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1 molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione S-transferase) and its subsequent excretion is regarded as an important detoxification pathway in animals. Aflatoxins are well recognized as a cause of liver cancer, but they have additional important toxic effects. Aflatoxin B1 is a potent hepatocarcinogenic and mutagenic mycotoxin of Aspergillus flavus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Aflatoxin B1 Antibody (NB100-2908). (Showing 1 - 1 of 1 FAQ).

1. My question was how sensitive is your most sensitive aflatoxin antibody? Say in a standard ELISA plate format. I could not find any info of that kind on your website.
   • We have one kit available, cat# KA1412, that can detect Aflatoxin levels of less than 1 ng/mL. The only other sensitivity information we have is that NB120-1017 can detect Aflatoxin when used at a concentration of 5ug/mL and NB100-2908 has a recommended dilution of 1:2000-1:10000 for use in ELISA.