Adenylate Kinase 1 Antibody (4A1)

Images

 
Western Blot: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylate Kinase 1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] - Immunofluorescent staining of HT29 cells using anti-Adenylate Kinase 1 mouse monoclonal antibody.
Flow Cytometry: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adenylate Kinase 1 ...read more
Western Blot: Adenylate Kinase 1 Antibody (4A1) [NBP1-47655] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adenylate Kinase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
4A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Adenylate Kinase 1 Antibody (4A1) Summary

Immunogen
Protein expressed in 293T cell transfected with human AK1 expression vector
Specificity
This antibody is specific for Homo sapiens adenylate kinase 1 (AK1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human, Primate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Adenylate Kinase 1 Antibody (4A1)

  • adenylate kinase 1
  • adenylate kinase isoenzyme 1
  • AK 1
  • ATP-AMP transphosphorylase 1
  • EC 2.7.4
  • EC 2.7.4.3
  • myokinase

Background

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol AK1
Entrez