Adenovirus Fiber Antibody (4D2)

Product Discontinued

Adenovirus Fiber Antibody (4D2) Summary

• Immunogen: UV-irradiated Ad2 virus
• Localization: Nuclear.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Reactivity Notes

Human reported in literature (PMID: 15756711). Hamster reactivity reported in scientific literature (see Ying B et all 2009). Mouse reactivity reported in scientific literature (see Jiang H et all 2009).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Adenovirus Fiber Antibody (4D2)

• Agnoprotein
• Fiber protein
• Fiber
• L5
• pIV
• Virus attachment protein

Background

Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenovirus Fiber Antibody (NB600-541)(3)

Publications using NB600-541

• Laspidea V, Puigdelloses M, Labiano S et al. Exploiting 4-1BB immune checkpoint to enhance the efficacy of oncolytic virotherapy for diffuse intrinsic pontine gliomas JCI insight 2022-04-08 [PMID: 35393952] (WB)
• Puigdelloses M, Garcia-Moure M, Labiano S Et Al. CD137 and PD-L1 targeting with immunovirotherapy induces a potent and durable antitumor immune response in glioblastoma models Journal for immunotherapy of cancer 2021-07-01 [PMID: 34281988] (WB)
• Dolan MJ, Frechter S, Bates AS et al. The oncolytic virus Delta-24-RGD elicits an antitumor effect in pediatric glioma and DIPG mouse models Nat Commun 2019-05-28 [PMID: 31138805] (WB, Human)

FAQs for Adenovirus Fiber Antibody (NB600-541). (Showing 1 - 1 of 1 FAQs).

1. Is there an accession number for the antigen recognized by this antibody? Is the epitope located on the N-terminus?
   • This antibody recognizes the N-terminus. There is no accession number associated with this target.