Adenovirus Fiber Antibody (4D2)

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Product Details

Summary
Reactivity Hu, Mu, Ha, ViSpecies Glossary
Applications WB, IF
Clone
4D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Adenovirus Fiber Antibody (4D2) Summary

Immunogen
UV-irradiated Ad2 virus
Localization
Nuclear.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunofluorescence 1:10 - 1:500

Reactivity Notes

Human reported in literature (PMID: 15756711). Hamster reactivity reported in scientific literature (see Ying B et all 2009). Mouse reactivity reported in scientific literature (see Jiang H et all 2009).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Adenovirus Fiber Antibody (4D2)

  • Agnoprotein
  • Fiber protein
  • Fiber
  • L5
  • pIV
  • Virus attachment protein

Background

Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Adenovirus Fiber Antibody (NB600-541). (Showing 1 - 1 of 1 FAQ).

  1. Is there an accession number for the antigen recognized by this antibody? Is the epitope located on the N-terminus?
    • This antibody recognizes the N-terminus. There is no accession number associated with this target.

Secondary Antibodies

 

Isotype Controls

Additional Adenovirus Fiber Products

Adenovirus Fiber NB600-541

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