Adenosine Deaminase/ADA Recombinant Protein Summary
A recombinant protein with a His-tag corresponding to amino acids 1 to 363 of Human Adenosine Deaminase/ADA
Source: E. coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL
|Details of Functionality
Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
Please see the vial label for concentration. If unlisted please contact technical services.
1. Prerapre a 1.5 ml reaction mix: the final concentrations are 53.3mM potassium phosphate, 0.045mM adenosine, 0.003% (w/v) bovine serum.
2. Add recombinant ADA protein with various concentrations (0.1ug, 0.2) in assay buffer.
3. Mix by inversion and record A260nm for approximately 5 minutes.
The purity of this protein is > 85% by SDS-PAGE. Molecular weight is 42.9 kDa (383aa), confirmed by MALDI-TOF
Alternate Names for Adenosine Deaminase/ADA Recombinant Protein
- adenine deaminase
- Adenosine aminohydrolase
- adenosine deaminase
- EC 18.104.22.168
ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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