Adenosine A2a R Antibody (7F6-G5-A2)

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Western Blot: Adenosine A2a R Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane ...read more
Immunocytochemistry/ Immunofluorescence: Adenosine A2a R Antibody (7F6-G5-A2) [NBP1-39474] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. ...read more
Immunohistochemistry-Paraffin: Adenosine A2a R Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of anti-ADORA2A antibody with human brain, putamen at concentration 5 ug/ml.
Flow Cytometry: Adenosine A2a R Antibody (7F6-G5-A2) [NBP1-39474] - Using the PE direct conjugate An intracellular stain was performed on SH-SY5Y cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a ...read more
Immunocytochemistry/ Immunofluorescence: Adenosine A2a R Antibody (7F6-G5-A2) [NBP1-39474] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% ...read more
Immunohistochemistry-Paraffin: Adenosine A2a R Antibody (7F6-G5-A2) [NBP1-39474] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at ...read more
Immunohistochemistry-Paraffin: Adenosine A2a R Antibody (7F6-G5-A2) [NBP1-39474] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at ...read more
Flow (Intracellular): Adenosine A2a R Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on hPBMCs with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control NBP2-14864. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, GP, RbSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
7F6-G5-A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Adenosine A2a R Antibody (7F6-G5-A2) Summary

Immunogen
Full length recombinant human Adenosine A2a R. [UniProt# P29274]
Epitope
Epitope mapped to SQPLPGER in the third intracellular loop (PMID 9822147).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ADORA2A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1-5 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1 -5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1 -5 ug/ml
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
ICC usage is reported in scientific literature (PMID: 25836920). Use in IHC-frozen secions reported in scientific literature (PMID 9822147).
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-39474 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Adenosine A2a R Antibody (7F6-G5-A2)

  • A2aR
  • adenosine A2 receptor
  • Adenosine A2a R
  • adenosine A2a receptor
  • Adenosine A2aR
  • adenosine receptor A2a
  • adenosine receptor subtype A2a
  • ADORA 2
  • ADORA2
  • ADORA2A
  • hA2aR
  • RDC8

Background

This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Adenosine A2a R Antibody (NBP1-39474)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: FLOW, Flow, ICC, ICC/IF, IHC-Fr, WB.


Filter By Application
FLOW
(1)
Flow
(1)
ICC
(1)
ICC/IF
(2)
IHC-Fr
(1)
WB
(5)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Rat
(2)
Porcine
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-39474 Applications Species
Mediavilla-Varela M, Castro J, Chiappori A et al. A Novel Antagonist of the Immune Checkpoint Protein Adenosine A2a Receptor Restores Tumor-Infiltrating Lymphocyte Activity in the Context of the Tumor Microenvironment. Neoplasia. 2017 Jun 02 [PMID: 28582704] (Flow, Human) Flow Human
Clancy-Thompson E, Perekslis TJ, Croteau W et al. Melanoma Induces, and Adenosine Suppresses, CXCR3-Cognate Chemokine Production and T-cell Infiltration of Lungs Bearing Metastatic-like Disease. Cancer Immunol Res. 2015 Aug 1 [PMID: 26048575] (FLOW, Mouse, Human)

Details:
Adenosine A2a R antibody (clone 7F6-G5-A2) was used for FLOW analysis of adenosine receptor A2A (ADORA2A) in murine B16 melanoma and human VMM12 melanoma cells (Figure 7).
FLOW Mouse, Human
Del Ry S, Moscato S, Bianchi F et al. Altered expression of connexin 43 and related molecular partners in a pig model of left ventricular dysfunction with and without dipyrydamole therapy Pharmacol. Res. 2015 Mar 30 [PMID: 25836920] (ICC/IF, Rat) ICC/IF Rat
Ribeiro AS, Fernandes VS, Orensanz LM, Martinez MP, Recio P, Martinez-Saenz A, Climent B, Arteaga JL, Garcia-Sacristan A, Prieto D, Hernandez M. Mechanisms involved in the adenosine-induced vasorelaxation to the pig prostatic small arteries. Purinergic Signal;7(4):413-25. 2011 Dec. [PMID: 21567127] (WB, Porcine) WB Porcine
Haschemi A, Wagner O, Marculescu R et al. Cross-regulation of carbon monoxide and the adenosine A2a receptor in macrophages. J Immunol. 2007 May 1 [PMID: 17442976] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Nguyen DK, Montesinos MC, Williams AJ et al. Th1 cytokines regulate adenosine receptors and their downstream signaling elements in human microvascular endothelial cells. J Immunol. 2003 Oct 15 [PMID: 14530318] (WB, Human)

Details:
Adenosine A2a R antibody (clone 7F6-G5-A2) was used for Western blot analysis of adenosine A2A receptors in Human Microvascular Endothelial Cells (HMVECs) which were treated overnight with IL-1, TNF-alpha, or IFN-gamma.
WB Human
Khoa ND, Montesinos MC, Reiss AB et al. Inflammatory cytokines regulate function and expression of adenosine A(2A) receptors in human monocytic THP-1 cells. J Immunol. 2001 Oct 1 [PMID: 11564822] (WB, Human)

Details:
Adenosine A2a R antibody (clone 7F6-G5-A2) was used for Western blot analysis of crude membrane protein lysates from THP-1 cells which were treated or not with IL1, TNF-alpha or IFN-gamma.
WB Human
Rosin DL, Robeva A, Woodard RL et al. Immunohistochemical localization of adenosine A2A receptors in the rat central nervous system. J Comp Neurol. 1998 Nov 16 [PMID: 9822147] (WB, ICC, IHC-Fr, Rat) WB, ICC, IHC-Fr Rat
Nguyen KD. Inflammatory Cytokines Regulate Adenosine A2A Receptor Expression, Function, and Desensitization. Book from NEW YORK UNIVERSITY. 2007

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Adenosine A2a R Antibody (NBP1-39474) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-39474AF405
Alexa Fluor 488 Labeled NBP1-39474AF488
Alexa Fluor 647 Labeled NBP1-39474AF647
Alexa Fluor 700 Labeled NBP1-39474AF700
Allophycocyanin Labeled NBP1-39474APC
DyLight 350 Labeled NBP1-39474UV
DyLight 405 Labeled NBP1-39474V
DyLight 488 Labeled NBP1-39474G
DyLight 680 Labeled NBP1-39474FR
DyLight 755 Labeled NBP1-39474IR
PE Labeled NBP1-39474PE
PerCP Labeled NBP1-39474PCP

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Bioinformatics

Gene Symbol ADORA2A
Entrez
Uniprot