Adenosine A1 R Antibody

Images

 
Western Blot: Adenosine A1 R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).
Immunocytochemistry/ Immunofluorescence: Adenosine A1 R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells.
Immunohistochemistry-Paraffin: Adenosine A1 R Antibody [NB300-549] - Analysis showing positive staining in the cytoplasm of Rat brain tissue (right) compared with a negative control in the absence of primary antibody ...read more
Flow Cytometry: Adenosine A1 R Antibody [NB300-549] - Flow cytometry analysis of Adenosine Receptor A1 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 ...read more
Immunocytochemistry/ Immunofluorescence: Adenosine A1 R Antibody [NB300-549] - ICC on dissociated rat hippocampal culture. Image courtesy of Janosch Heller, University College London.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Adenosine A1 R Antibody Summary

Immunogen
Synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.
Localization
Cell Membrane
Specificity
Detects Adenosine Receptor A1. This does not detect other AR subtypes.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADORA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
ICC usage was reported by customer review.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB300-549 in the following applications:

Publications
Read Publications using
NB300-549 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 18948751). Rat from customer review.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Adenosine A1 R Antibody

  • Adenosine A1 R
  • adenosine A1 receptor
  • Adenosine A1R
  • ADORA 1
  • ADORA1
  • RDC7
  • RDC7adenosine receptor A1

Background

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission.The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Adenosine A1 R Antibody (NB300-549)(4)

Review for Adenosine A1 R Antibody (NB300-549) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB300-549:
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Adenosine A1 R NB300-549
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reviewed by:
Anony- mous
ICC Rat 08/27/2014
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Summary

ApplicationImmunocytochemistry
SpeciesRat
Special ApplicationsReview courtesy of Janosch Heller, University College London
FileView PDF

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Anony- mous
08/27/2014
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol ADORA1
Entrez
Uniprot