ADE2 Antibody (1B4)

Images

 
Western Blot: ADE2 Antibody (1B4) [NBP2-02817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: ADE2 Antibody (1B4) [NBP2-02817] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ADE2.
Flow Cytometry: ADE2 Antibody (1B4) [NBP2-02817] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ADE2 antibody, and then analyzed by ...read more
Flow Cytometry: ADE2 Antibody (1B4) [NBP2-02817] - Analysis of Jurkat cells, using anti-ADE2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
1B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ADE2 Antibody (1B4) Summary

Immunogen
Full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PAICS
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ADE2 Antibody (1B4)

  • ADE2
  • AIR carboxylase
  • AIRCADE2H1
  • DKFZp781N1372
  • MGC1343
  • MGC5024
  • multifunctional protein ADE2
  • PAIS
  • phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazolesuccinocarboxamide synthetase
  • SAICAR synthetase

Background

This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAICS
Entrez