ADAM8 RNAi

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications RNAi, RNAi SP

Order Details

ADAM8 RNAi Summary

Specificity
ADAM metallopeptidase domain 8 (ADAM8), mRNA
Gene
ADAM8

Applications/Dilutions

Application Notes
This RNAi causes protein knockdown.
Reviewed Applications
Read 1 Review rated 5
using
H00000101-R01 in the following application:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ADAM8 RNAi

  • a disintegrin and metalloproteinase domain 8
  • ADAM 8
  • ADAM metallopeptidase domain 8
  • ADAM8
  • CD156a antigen
  • CD156a
  • CD156human leukocyte differentiation antigen
  • Cell surface antigen MS2
  • EC 3.4.24
  • EC 3.4.24.-
  • MGC134985
  • MS2
  • MS2disintegrin and metalloproteinase domain-containing protein 8

Background

Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene.  The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.  Chimera RNAi has many advantages over the conventional siRNAs.  First, it has been demonstrated to have reliable knock-down for over 10,000 human genes.  Because the human genome is composed of an intricate, genetic network, chimera RNAi’s unique design has successfully obviated the off-target effects including microRNA-based influence.  Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected.  Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.

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Publications for ADAM8 RNAi (H00000101-R01) (0)

There are no publications for ADAM8 RNAi (H00000101-R01).
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Review for ADAM8 RNAi (H00000101-R01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00000101-R01:
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Western Blot ADAM8 H00000101-R01
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reviewed by:
Anonymous
WB Human 11/18/2014
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Summary

ApplicationWestern Blot
Sample TestedPSN1 whole cell lysate
SpeciesHuman
LotA20141014-190

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7074
Secondary Concentration1:5000

Details

Detection NotesECL, 1 min exposure show clear band

Comments

CommentsCells were transfected with ADAM8 RNAi for 48 hours, then whole cell lysates were collected. In western blot assy, ADAM8 protein levels were decreased for about 50%, which means RNAi worked quite well in the transfection.

Product General Protocols

View specific protocols for ADAM8 RNAi (H00000101-R01): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ADAM8 RNAi (H00000101-R01) (0)

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Anonymous
11/18/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ADAM8
Entrez