This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for ADAM8 RNAi
a disintegrin and metalloproteinase domain 8
ADAM metallopeptidase domain 8
CD156human leukocyte differentiation antigen
Cell surface antigen MS2
MS2disintegrin and metalloproteinase domain-containing protein 8
Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene. The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo. Chimera RNAi has many advantages over the conventional siRNAs. First, it has been demonstrated to have reliable knock-down for over 10,000 human genes. Because the human genome is composed of an intricate, genetic network, chimera RNAis unique design has successfully obviated the off-target effects including microRNA-based influence. Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected. Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.
2% cold fish geletin in TBS buffer, room temperature for 1hour
1:1000 overnight at 4C in blocking buffer
Goat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#
Cell Signaling, #7074
ECL, 1 min exposure show clear band
Cells were transfected with ADAM8 RNAi for 48 hours, then whole cell lysates were collected. In western blot assy, ADAM8 protein levels were decreased for about 50%, which means RNAi worked quite well in the transfection.
Product General Protocols
View specific protocols for ADAM8 RNAi (H00000101-R01):