actin, gamma 2 Antibody

Images

 
Western Blot: actin, gamma 2 Antibody [NB100-91649] - Lane1:DLD cell lysate. Lane2:Rat brain tissue. Lane 3: Mouse brain tissue lysate
Immunohistochemistry-Paraffin: actin, gamma 2 Antibody [NB100-91649] - Paraffin-embedded human colon carcinoma tissue at 1:50
Immunohistochemistry-Paraffin: actin, gamma 2 Antibody [NB100-91649] - Analysis in paraffin-embedded human breast carinoma tissue at 1:100

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-91649
    • Availability
      Product Discontinued

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actin, gamma 2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to the N-terminal of Human actin, gamma 2.
Specificity
actin, gamma 2 Antibody (E2) detects endogenous levels of Actin, gamma-enteric smooth muscle protein.
Clonality
Polyclonal
Host
Rabbit
Gene
ACTG2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
actin, gamma 2 Lysate (NBL1-07274)
Publications
Read Publications using
NB100-91649 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for actin, gamma 2 Antibody

  • ACT
  • ACTA3alpha-actin 3
  • ACTE
  • actin, gamma 2, smooth muscle, enteric
  • actin, gamma-enteric smooth muscle
  • ACTL3
  • ACTSGactin-like protein
  • alpha-actin-3
  • gamma-2-actin
  • smooth muscle gamma actin
  • Smooth muscle gamma-actin

Background

All eukaryotic cells express actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. alpha actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for actin, gamma 2 Antibody (NB100-91649)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACTG2
Entrez
Uniprot