Actin Antibody (AC-40)

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Western Blot: Actin Antibody (AC-40) [NBP1-41294]
Immunohistochemistry-Frozen: Actin Antibody (AC-40) [NBP1-41294]

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-41294
    • Availability
      Product Discontinued

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Actin Antibody (AC-40) Summary

Immunogen
Synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 4 ug/ml
Reviewed Applications
Read 1 Review rated 5
using
NBP1-41294 in the following applications:

Publications
Read Publications using NBP1-41294.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 1.2% sodium acetate, and 2mg BSA
Preservative
0.01 mg Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml distilled water.

Alternate Names for Actin Antibody (AC-40)

  • ACTA
  • Actin
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Actin Antibody (NBP1-41294)(2)

Review for Actin Antibody (NBP1-41294) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-41294:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Actin NBP1-41294
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reviewed by:
Ronald Miller
WB Human 05/29/2014
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Summary

ApplicationWestern Blot
Sample TestedYoung and senescent cells
SpeciesHuman
CommentsThis actin antibody works very well. The signal here is saturated. Can be used in a diluted format with lesser amount of protein.

Blocking

Blocking Details5% BSA in 1X PBS

Primary Anitbody

Dilution Ratio1:2000 in 1X PBS with troton X-100 (0.1%) at 4 degree O/N

Secondary Antibody

Secondary DescriptionGoat anti mouse-HRP conjugated
Secondary Manufacturer Cat#A24518
Secondary Concentration1:10,000

Details

Detection NotesECL, 10 seconds exposure with film

Comments

CommentsThis actin antibody works very well. The signal here is saturated. Can be used in a diluted format with lesser amount of protein.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Actin Antibody (NBP1-41294). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NBP1-41294

Bioinformatics Tool for Actin Antibody (NBP1-41294)

Discover related pathways, diseases and genes to Actin Antibody (NBP1-41294). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Actin Antibody (NBP1-41294)

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Pathways for Actin Antibody (NBP1-41294)

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PTMs for Actin Antibody (NBP1-41294)

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Research Areas for Actin Antibody (NBP1-41294)

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Blogs on Actin. Showing 1-10 of 16 blog posts - Show all blog posts.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

CRISPR/Cas9: Keep your friends close, but your viruses closer
"CRISPR", or clustered regularly interspaced short palindromic repeats, is an ancient bacterial mechanism that prevents the invasion of foreign pathogens to a host organism.  Specifically, the CRISPR sequence has been identified as a si...  Read full blog post.

Alpha-actin/ACTA1 - A skeletal muscle isoform mutated in various myopathies
Actin is an abundant cytoskeletal protein involved in a variety of cellular processes such as cell motility, cell division, and muscle contraction. Actin monomers assemble into filaments and can provide a track for transport of cargo by the molecul...  Read full blog post.

Alpha-Adducin - Assembling the cytoskeleton meshwork that underlies the plasma membrane
The structure and organization of the plasma membrane is maintained by an underlying network of cytoskeletal proteins including actin and spectrin. Adducin, a member of this protein network, binds to bundles and caps actin filaments and links them...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Actin Dynamics and Endocytic Trafficking
Actin is a ubiquitous and an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes. It is abundant protein whose monomers polymerize into polarized actin filaments, within epithelial cells. Filamentous acti...  Read full blog post.

Beta Actin is a Key Player in Embryonic Development and Cell Motility
Actins are an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes, including cell migration, division, and regulation of gene expression. These functions are attributed to the ability of actin to form fil...  Read full blog post.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Recent Reviews

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Ronald Miller
05/29/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTA1
Entrez
Uniprot