Actin Alpha 1 Skeletal Muscle Antibody

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Western Blot: Actin Alpha 1 Skeletal Muscle Antibody [NB100-91648] - Western blot (WB) analyzes of Actin Alpha 1 Skeletal Muscle (D3) antibody in extracts from LOVO rat muscle cells.
Immunohistochemistry: Actin Alpha 1 Skeletal Muscle Antibody [NB100-91648] - Immunohistochemistry (IHC) analyzes of Actin-Actin Alpha 1 Skeletal Muscle (D3) antibody in paraffin-embedded human muscle tissue.

Order Details


    • Catalog Number
      NB100-91648
    • Availability
      Product Discontinued

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Actin Alpha 1 Skeletal Muscle Antibody Summary

Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Actin-alpha1.
Specificity
Actin Alpha 1 Skeletal Muscle detects endogenous levels of Actin Alpha 1 Skeletal Muscle.
Clonality
Polyclonal
Host
Rabbit
Gene
ACTA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of Actin-alpha -1 (D3) pAb in extracts from LOVO rat muscle cells.
Immunohistochemistry (IHC) analyzes of Actin-alpha -1 (D3) pAb in paraffin-embedded human muscle tissue.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-91648 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Actin Alpha 1 Skeletal Muscle Antibody

  • ACTA
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Background

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Actin Alpha 1 Skeletal Muscle Antibody (NB100-91648)(3)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for Actin Alpha 1 Skeletal Muscle Antibody (NB100-91648)

Discover related pathways, diseases and genes to Actin Alpha 1 Skeletal Muscle Antibody (NB100-91648). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Actin Alpha 1 Skeletal Muscle Antibody (NB100-91648)

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PTMs for Actin Alpha 1 Skeletal Muscle Antibody (NB100-91648)

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Blogs on Actin Alpha 1 Skeletal Muscle.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

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Bioinformatics

Gene Symbol ACTA1
Entrez
Uniprot