This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation inbuffered formalin can destroy the epitope. It is recommended that this product be used on frozen tissue sectionsor specimens. The optimal conditions should be determined by the individual laboratory. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26567220)
Read Publication using NB120-7799 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 26567220). Cross-reacts with sheep, bovine, guinea pig, rat, frog, snake and carp. Not yet tested in other species.
Alternate Names for Actin Alpha 1 Skeletal Muscle Antibody (5C5)
actin, alpha 1, skeletal muscle
alpha skeletal muscle actin
alpha skeletal muscle
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.