Actin Alpha 1 Skeletal Muscle Antibody (5C5)

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Immunohistochemistry: Actin Alpha 1 Skeletal Muscle Antibody (5C5) [NB120-7799] - Formalin fixed paraffin embedded human skeletal muscle.

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
5C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Actin Alpha 1 Skeletal Muscle Antibody (5C5) Summary

Immunogen
The purified rabbit striated muscle actin.
Specificity
Monoclonal anti-ALPHA-sarcomeric actin has been used as a marker for rhabdomyosarcoma. This reacts with a-skeletal and a-cardiac muscle actins. It does not react with smooth muscle tissue.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:50
Application Notes
This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation inbuffered formalin can destroy the epitope. It is recommended that this product be used on frozen tissue sectionsor specimens. The optimal conditions should be determined by the individual laboratory. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26567220)
Publications
Read Publication using
NB120-7799 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26567220). Cross-reacts with sheep, bovine, guinea pig, rat, frog, snake and carp. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Ascites
Preservative
Sodium Azide
Purity
Unpurified

Alternate Names for Actin Alpha 1 Skeletal Muscle Antibody (5C5)

  • ACTA
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Background

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Actin Alpha 1 Skeletal Muscle Antibody (NB120-7799)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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(1)
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Discover related pathways, diseases and genes to Actin Alpha 1 Skeletal Muscle Antibody (NB120-7799). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Actin Alpha 1 Skeletal Muscle.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

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Bioinformatics

Gene Symbol ACTA1
Entrez