Actin Alpha 1 Skeletal Muscle Antibody (3B3)

Images

 
Immunohistochemistry-Paraffin: Actin Alpha 1 Skeletal Muscle Antibody (3B3) [NBP1-97723] - Paraffin sections of human striated skeletal muscle (above) and striated muscle (below) from human tongue immunostained with 3B3 ...read more
Immunohistochemistry-Paraffin: Actin Alpha 1 Skeletal Muscle Antibody (3B3) [NBP1-97723]

Product Details

Summary
Reactivity Hu, Rt, Po, Gt, RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
3B3
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

Actin Alpha 1 Skeletal Muscle Antibody (3B3) Summary

Immunogen
Clone 3B3 is a mouse monoclonal IgG1 antibody derived by fusion of NS0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Specificity
The monoclonal antibody a-SKA, clone 3B3 is an antibody against alpha skeletal actin, the antibody is highly specific for alpha-skeletal actin and does not cross react with other actin isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Reactivity Notes

The epitope recognized by Clone 3B3 is highly conserved and the antibody is therefore expected to react with amny other species.

Alternate Names for Actin Alpha 1 Skeletal Muscle Antibody (3B3)

  • ACTA
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Actin Alpha 1 Skeletal Muscle.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol ACTA1

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