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ACP2 RNAi

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Western Blot: ACP2 RNAi [H00000053-R01V] - Analysis of ACP2 over-expressed 293 cell line. Lane 2 cotransfected with ACP2 Validated Chimera RNAi. Lane 1 non-transfected control. Blot probed with H00000053-M01. GAPDH ( 36.1 kDa ) used as loading control.
Western Blot: ACP2 RNAi [H00000053-R01V] - Analysis of ACP2 over-expressed 293 cell line. Lane 2 cotransfected with ACP2 Validated Chimera RNAi. Lane 1 non-transfected control. Blot probed with H00000053-M01. GAPDH ( ...read more
RNAi sequence position: ACP2 RNAi [H00000053-R01V]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, RNAi, RNAi SP

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ACP2 RNAi Summary

Specificity
Homo sapiens acid phosphatase 2, lysosomal, mRNA
Gene
ACP2

Applications/Dilutions

Application Notes
This RNAi causes effective protein knockdown which has been antibody validated.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ACP2 RNAi

  • acid phosphatase 2, lysosomal
  • EC 3.1.3.2
  • LAP
  • lysosomal acid phosphatase

Background

Position of the Chimera RNAi. The related RNAi products listed were designed from different accesion number but sharing the same RNAi sequence. Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene. The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo. Chimera RNAi has many advantages over the conventional siRNAs. First, it has been demonstrated to have reliable knock-down for over 10,000 human genes. Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence. Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected. Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.

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Western Blot: LAP3 Antibody [NBP1-82847] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: LAP3 Antibody [NBP1-82847] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & midbody.
Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847] - Staining of human skeletal muscle shows low expression as expected.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
ASRGL1 Antibody
(CL1679)
NBP2-42378
Western Blot: ASRGL1 Antibody (CL1679) [NBP2-42378] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry: ASRGL1 Antibody (CL1679) [NBP2-42378] - Staining of human liver shows absence of immunoreactivity (negative control).
Immunohistochemistry: ASRGL1 Antibody (CL1679) [NBP2-42378] - Staining of human endometrial cancer shows strong immunoreactivity in tumor cells.
Species: Hu
Applications: WB, IHC, IHC-P
TGF-beta 1
[Unconjugated]
240-B
Epithelial to Mesenchymal Transition (EMT) was induced in the A549 human lung carcinoma cell line with cell culture media supplemented with Recombinant Human (rh) TGF-beta 1 (Catalog # 240-B). Control cells were cultured without rhTGF-beta 1. EMT induction was confirmed at 48 h by flow cytometric staining for E-Cadherin (filled; Catalog # FAB18381P), an epithelial cell marker, or an isotype control (Catalog # IC0041P). TGF-beta 1 decreased the expression of E-Cadherin.
1 μg/lane of Recombinant Human TGF-beta 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.
Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.
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Species: Hu
Prostatic Acid
Phosphatase/ACPP Anti ...
AF6240
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostatic Acid Phosphatase/ACPP at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Prostatic Acid Phosphatase/ACPP was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Recombinant Human Prostatic Acid Phosphatase/ACPP protein was serially diluted 2-fold and captured by Mouse Anti-Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB62401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Species: Hu
Applications: WB, ELISA, IHC
PAG3 Antibody
NBP2-24614
Western Blot: PAG3 Antibody [NBP2-24614] - Analysis of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified this antibody. 1.0 ug/ml.
Species: Hu, Mu, Rt, Op, Pm
Applications: WB
Reg3A Antibody
NBP2-24763
Western Blot: Reg3a Antibody [NBP2-24763] - Human intestine lysate probed with REG3A antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: Reg3a Antibody [NBP2-24763] - Formalin-fixed, paraffin-embedded human intestine stained with REG3A antibody (2.5 ug/ml), peroxidase-conjugate and DAB chromogen.
(1 Publication)
Species: Hu, Pm
Applications: WB, IHC-P
PAPOLA Knockout
293T Cell Lysate
NBP2-65899
PAPOLA Knockout 293T Cell Lysate
Species: Hu
MRPS30 Antibody
NBP1-87343
Western Blot: MRPS30 Antibody [NBP1-87343] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: MRPS30 Antibody [NBP1-87343] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Western Blot: MRPS30 Antibody [NBP1-87343] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
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Applications: WB, IHC, IHC-P
ASAP1 [p Tyr782]
Antibody
NBP1-77933
Immunocytochemistry/Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.
Immunohistochemistry: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Species: Hu, Mu, Rt, Bv, Ca, Ch, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
TUSC2 Antibody
NBP1-36999
TUSC2 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA
PDAP1 Knockout
293T Cell Lysate
NBP2-65749
PDAP1 Knockout 293T Cell Lysate
Species: Hu
GGT1 Antibody (1F9)
H00002678-M01
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Immunohistochemistry-Paraffin: GGT1 Antibody (1F9) [H00002678-M01] - Analysis of monoclonal antibody to GGT1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Immunoprecipitation: GGT1 Antibody (1F9) [H00002678-M01] - Analysis of GGT1 transfected lysate using anti-GGT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GGT1 MaxPab rabbit polyclonal antibody.
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Species: Hu, Mu
Applications: WB, ELISA, IHC-P, IP
Alkaline
Phosphatase/ALPP Anti ...
NB110-3638
Western Blot: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review.
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic Induced Epitope Retrieval (PIER) was used.
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SMG1 Antibody
NB100-2320
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Western Blot: SMG1 Antibody [NB100-2320] - Detection of Human SMG1 on HeLa whole cell lysate using NB100-2320. IP of SMG1 was successful when using affinity purified rabbit anti-SMG1 antibodies NB100-2321 and another SMG1 antibody.
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ATRNL1 Antibody
NBP1-89131
Immunocytochemistry/Immunofluorescence: ATRNL1 Antibody [NBP1-89131] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: ATRNL1 Antibody [NBP1-89131] - Staining of human small intestine shows strong cytoplasmic and membranous positivity.
Immunohistochemistry: ATRNL1 Antibody [NBP1-89131] - Staining of human small intestine shows strong cytoplasmic and membranous positivity.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NAT10 Antibody
NBP2-32263
Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Species: Hu
Applications: WB, IHC, IP
PDLIM3 Antibody
NBP1-89134
Immunocytochemistry/Immunofluorescence: PDLIM3 Antibody [NBP1-89134] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining of human skeletal muscle shows high expression.
Immunohistochemistry-Paraffin: PDLIM3 Antibody [NBP1-89134] - Staining of human cerebral cortex shows low expression as expected.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
ACP2 RNAi
H00000053-R01V
ACP2 RNAi
Species: Hu
Applications: WB, RNAi, RNAi SP

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Bioinformatics

Gene Symbol ACP2