Aconitase 2 Antibody (3G8)

Images

 
Western Blot: Aconitase 2 Antibody (3G8) [NBP2-02241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aconitase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (3G8) [NBP2-02241] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Ovary tissue using anti-Aconitase 2 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody (3G8) [NBP2-02241] - Immunofluorescent staining of HepG2 cells using anti-Aconitase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Aconitase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody (3G8) [NBP2-02241] - Staining of paraffin-embedded Kidney tissue using anti-Aconitase 2 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Aconitase 2 Antibody (3G8) Summary

Immunogen
Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Reviewed Applications
Read 1 Review rated 4
using
NBP2-02241 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Aconitase 2 Antibody (3G8)

  • aconitase 2, mitochondrial
  • Aconitase
  • aconitate hydratase, mitochondrial
  • ACONM
  • Citrate hydro-lyase
  • EC 4.2.1
  • EC 4.2.1.3
  • MGC20605
  • MGC33908

Background

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aconitase 2 Antibody (NBP2-02241) (0)

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Review for Aconitase 2 Antibody (NBP2-02241) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-02241:
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Rat 12/29/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23271731
SpeciesRat

Blocking

Blocking DetailsSee PMID 23271731

Details

Detection NotesSee PMID 23271731

Comments

CommentsPublished in PMID 23271731.Independent review from antibodyresource.com

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Video Protocols

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ICC/IF Video Protocol

FAQs for Aconitase 2 Antibody (NBP2-02241) (0)

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Secondary Antibodies

 

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Anonymous
12/29/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol ACO2
Entrez