Western Blot: Acetylated Lysine Antibody (15G10) [NB100-78428] - Untreated Hela cells (lane 1) and sodium butyrate-treated Hela cells (24 hr treatment, lane 2) were lysed and cell extracts resolved by electrophoresis, ...read more
Immunocytochemistry/ Immunofluorescence: Acetylated Lysine Antibody (15G10) [NB100-78428] - Untreated Hela cells (Upper Panel), or overnight nocodazole treated Hela cells (Lower Panel) stained with purified mouse ...read more
This antibody is provided in phosphate-buffered solution, pH 7.2
0.09% Sodium Azide
Protein A or G purified
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 mug/ml. It is recommended that the reagent be titrated for optimal performance for each application. Immunoprecipitation reactivity per customer review.
Read 1 Review rated 3 using NB100-78428 in the following application:
Alternate Names for Acetylated Lysine Antibody (15G10)
Proteins are reversibly and dynamically acetylated on the epsilon-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetytransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Acetylated Lysine Antibody (NB100-78428)(1)
FAQs for Acetylated Lysine Antibody (NB100-78428). (Showing 1 - 1 of 1 FAQ).
For Acetylated Lysine Antibody, NB100-78428, can you please provide the exact dilution on this format like 1:50...1:500 etc ? Also, is it possible to use a DAB based chromogen with it rather than fluorescence?
Acetylated Lysine antibody (15G10) [NB100-78428] was provided at concentration of 0.5 mg/ml, therefore, its dilutions may also be expressed as: Western Blot 1 ug/ml = 1:500; Immunocytochemistry/Immunofluorescence 1-4 ug/ml = 1:125 - 1:500. Yes, the detection may be performed via DAB method also provided the customer use a HRP- labelled anti-Mouse IgG secondary antibody or an appropriate anti-Mouse Detection kit for the post primary analysis.