Acetylated Lysine Antibody (15G10)

Images

 
Western Blot: Acetylated Lysine Antibody (15G10) [NB100-78428] - Untreated Hela cells (lane 1) and sodium butyrate-treated Hela cells (24 hr treatment, lane 2) were lysed and cell extracts resolved by electrophoresis, ...read more
Immunocytochemistry/ Immunofluorescence: Acetylated Lysine Antibody (15G10) [NB100-78428] - Untreated Hela cells (Upper Panel), or overnight nocodazole treated Hela cells (Lower Panel) stained with purified mouse ...read more

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ICC/IF, IP
Clone
15G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Acetylated Lysine Antibody (15G10) Summary

Immunogen
Acetylated protein mixture
Specificity
Acetylated Lysine (15G10)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 mug/ml. It is recommended that the reagent be titrated for optimal performance for each application. Immunoprecipitation reactivity per customer review.
Reviewed Applications
Read 1 Review rated 3
using
NB100-78428 in the following applications:

Publications
Read Publication using NB100-78428.

Reactivity Notes

Acetylated lysine residues.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Acetylated Lysine Antibody (15G10)

  • acetyl Lysine
  • Lysine

Background

Proteins are reversibly and dynamically acetylated on the epsilon-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetytransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acetylated Lysine Antibody (NB100-78428)(1)

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Acetylated Lysine Antibody (NB100-78428) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IP.

Reviews using NB100-78428:
Filter by Applications
IP
(1)
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Filter by Species
Other
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IP Other 02/04/2009
View

Summary

ApplicationImmunoprecipitation
Sample TestedMammary Epithial Cell Line
SpeciesOther
LotB107560
CommentsNot as sensitive for WB as Santa Cruz Mouse Monoclonal(AKL5C1) even at twice the concentration (mg/ml). Lysate Amount: 5MG, Ab Dilution Ratio: 1/20, Protein Capture: Protein G

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: 100mM NaPhosphate pH8.2 0.01% Tween, Dilution Ratio: 1/500, Incubation Time/Temp: 4 hrs at 4C

Comments

CommentsNot as sensitive for WB as Santa Cruz Mouse Monoclonal(AKL5C1) even at twice the concentration (mg/ml). Lysate Amount: 5MG, Ab Dilution Ratio: 1/20, Protein Capture: Protein G

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Acetylated Lysine Antibody (NB100-78428). (Showing 1 - 1 of 1 FAQ).

  1. For Acetylated Lysine Antibody, NB100-78428, can you please provide the exact dilution on this format like 1:50...1:500 etc ? Also, is it possible to use a DAB based chromogen with it rather than fluorescence?
    • Acetylated Lysine antibody (15G10) [NB100-78428] was provided at concentration of 0.5 mg/ml, therefore, its dilutions may also be expressed as: Western Blot 1 ug/ml = 1:500; Immunocytochemistry/Immunofluorescence 1-4 ug/ml = 1:125 - 1:500. Yes, the detection may be performed via DAB method also provided the customer use a HRP- labelled anti-Mouse IgG secondary antibody or an appropriate anti-Mouse Detection kit for the post primary analysis.

Secondary Antibodies

 

Isotype Controls

Additional Acetylated Lysine Products

Acetylated Lysine NB100-78428

Blogs on Acetylated Lysine

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Anonymous
02/04/2009
Application: IP
Species: Other