Acetylated Lysine Antibody (15G10)


Western Blot: Acetylated Lysine Antibody (15G10) [NB100-78428] - Untreated Hela cells (lane 1) and sodium butyrate-treated Hela cells (24 hr treatment, lane 2) were lysed and cell extracts resolved by electrophoresis, more
Immunocytochemistry/ Immunofluorescence: Acetylated Lysine Antibody (15G10) [NB100-78428] - Untreated Hela cells (Upper Panel), or overnight nocodazole treated Hela cells (Lower Panel) stained with purified mouse more

Product Details

Reactivity AllSpecies Glossary
Applications WB, ICC/IF, IP
0.5 mg/ml

Acetylated Lysine Antibody (15G10) Summary

Acetylated protein mixture
Acetylated Lysine (15G10)
IgG2b Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
This antibody is provided in phosphate-buffered solution, pH 7.2
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 mug/ml. It is recommended that the reagent be titrated for optimal performance for each application. Immunoprecipitation reactivity per customer review.
Reviewed Applications
Read 1 Review rated 3
NB100-78428 in the following application:

Read Publication using NB100-78428.

Reactivity Notes

Acetylated lysine residues.

Alternate Names for Acetylated Lysine Antibody (15G10)

  • acetyl Lysine
  • Lysine


Proteins are reversibly and dynamically acetylated on the epsilon-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetytransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acetylated Lysine Antibody (NB100-78428)(1)

We have publications tested in 1 application: WB.

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Reviews for Acetylated Lysine Antibody (NB100-78428) (1) 31

Average Rating: 3
(Based on 1 reviews)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IP.

Reviews using NB100-78428:
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Images Ratings Applications Species Date Details
reviewed by:
IP Other 02/04/2009


Sample TestedMammary Epithial Cell Line
CommentsNot as sensitive for WB as Santa Cruz Mouse Monoclonal(AKL5C1) even at twice the concentration (mg/ml). Lysate Amount: 5MG, Ab Dilution Ratio: 1/20, Protein Capture: Protein G

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: 100mM NaPhosphate pH8.2 0.01% Tween, Dilution Ratio: 1/500, Incubation Time/Temp: 4 hrs at 4C


CommentsNot as sensitive for WB as Santa Cruz Mouse Monoclonal(AKL5C1) even at twice the concentration (mg/ml). Lysate Amount: 5MG, Ab Dilution Ratio: 1/20, Protein Capture: Protein G

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Acetylated Lysine Antibody (NB100-78428). (Showing 1 - 1 of 1 FAQ).

  1. For Acetylated Lysine Antibody, NB100-78428, can you please provide the exact dilution on this format like 1:50...1:500 etc ? Also, is it possible to use a DAB based chromogen with it rather than fluorescence?
    • Acetylated Lysine antibody (15G10) [NB100-78428] was provided at concentration of 0.5 mg/ml, therefore, its dilutions may also be expressed as: Western Blot 1 ug/ml = 1:500; Immunocytochemistry/Immunofluorescence 1-4 ug/ml = 1:125 - 1:500. Yes, the detection may be performed via DAB method also provided the customer use a HRP- labelled anti-Mouse IgG secondary antibody or an appropriate anti-Mouse Detection kit for the post primary analysis.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-78428AF405
Alexa Fluor (R) 488 Labeled NB100-78428AF488
Alexa Fluor (R) 647 Labeled NB100-78428AF647
Alexa Fluor (R) 700 Labeled NB100-78428AF700
Allophycocyanin Labeled NB100-78428APC
DyLight 350 Labeled NB100-78428UV
DyLight 405 Labeled NB100-78428V
DyLight 405LS Labeled NB100-78428V3
DyLight 488 Labeled NB100-78428G
DyLight 680 Labeled NB100-78428FR
DyLight 755 Labeled NB100-78428IR
PE Labeled NB100-78428PE
PerCP Labeled NB100-78428PCP

Additional Acetylated Lysine Antibody (15G10) Products

Acetylated Lysine NB100-78428

Blogs on Acetylated Lysine

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