Recombinant Human ACAT1 Protein

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SDS-Page: Recombinant Human ACAT1 Protein [NBP2-22664]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human ACAT1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 34-427 of Human ACAT1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSVSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNK IHMGSCAENT AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVIPVTV TVKGQPDVVV KEDEEYKRVD FSKVPKLKTV FQKENGTVTA ANASTLNDGA AALVLMTADA AKRLNVTPLA RIVAFADAAV EPIDFPIAPV YAASMVLKDV GLKKEDIAMW EVNEAFSLVV LANIKMLEID PQKVNINGGA VSLGHPIGMS GARIVGHLTH ALKQGEYGLA SICNGGGGAS AMLIQKL

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ACAT1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
43.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human ACAT1 Protein

  • ACAT
  • acetoacetyl Coenzyme A thiolase
  • Acetoacetyl-CoA thiolase
  • acetyl-CoA acetyltransferase 1
  • acetyl-CoA acetyltransferase, mitochondrial
  • acetyl-Coenzyme A acetyltransferase 1
  • EC 2.3.1
  • EC 2.3.1.9
  • MAT
  • mitochondrial acetoacetyl-CoA thiolase
  • T2
  • THIL

Background

ACAT1, also known as acetoacetyl-CoA thiolase, is an enzyme belongs to belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. ACAT1 is an acetyl-CoA C-acetyltransferase enzyme. This enzyme catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. It plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Recombinant human ACAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Array NBP2-22664

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Bioinformatics

Gene Symbol ACAT1