AbSelect TCS Purification Antibody Purification Kit

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Summary

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AbSelect TCS Purification Antibody Purification Kit Summary

Description
Antibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies.

AbSelect TCS is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from hybridoma supernatants. The method involves the capture of the antibody on the AbSelect TCS resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.

The components of the AbSelect TCS are fully compatible with the Lightning-Link antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.

Benefits:
  • All components and buffers supplied
  • Purify antibody from 10 - 50 mls of hybridoma TCS
Kit Type
Antibody Purification Kit

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1 vial of Neutralization buffer
  2. 1 or 3 purification column(s)
  3. 1 or 3 vials of AbSelect TCS Protein A resin
  4. 1 bottle of 10x Binding buffer
  5. 1 bottle of Washing buffer
  6. 1 bottle of Elution buffer
  7. 1 or 3 concentrator spin columns and collection tubes

Notes

AbSelect® is a registered trademark of Innova Biosciences.

Background

Antibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies. The components of the AbSelect TCS are fully compatible with the Lightning-Link antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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