AbSelect Antibody Purification Kit

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Summary

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AbSelect Antibody Purification Kit Summary

Description
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions. The AbSelect Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum.

The antibody to be purified or cleaned up ideally is in a volume of 100 ul to 0.5 ml. Up to 500 ug of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.

The components of the AbSelect kit are fully compatible with the Lightning-Link antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels.
Kit Type
Antibody Purification Kit

Applications/Dilutions

Reviewed Applications
Read 1 Review rated 5
using
860-0005 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1 or 3 spin cartridge / collecting tube assemblies
  2. 1 vial of 10x Binding buffer
  3. 1 or 3 vials of AbSelect Protein A resin
  4. 1 vial of Elution buffer
  5. 1 vial of Neutralization buffer
  6. 1 vial of Washing buffer
  7. 4 or 12 additional collecting tubes

Notes

AbSelect® is a registered trademark of Innova Biosciences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Review for AbSelect Kit (860-0005) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

Reviews using 860-0005:
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ELISA
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Images Ratings Applications Species Date Details
 
reviewed by:
Alissa Taylor
ELISA Human 09/24/2014
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Summary

ApplicationELISA
Sample Testedcommerically available IFN antibody
SpeciesHuman

FAQs for AbSelect Kit (860-0005). (Showing 1 - 2 of 2 FAQ).

  1. Can AbSelect Antibody Purification Kits be used for purification of other proteins in addition to antibodies?
    • No, the AbSelect Antibody Purification Kit is not suitable for purifying proteins other than antibodies. This kit uses a Protein A resin. Protein A binds to antibodies but not other proteins. The antibody molecules are therefore bound to the resin, while any other proteins in the sample are simply washed through in the wash step, and hence are removed. The antibody is then detached from the resin in the elution step. Therefore, you should not attempt to purify anything other than antibodies using an AbSelect kit. However, you may be interested in one of our Antibody Concentration and Clean Up Kits, catalog numbers 861-0010 (3 columns) and 861-0005 (1 column). This product, in contrast to AbSelect, purifies by size: the sample is passed through a filter with a 10 kDa molecular weight cut-off. Therefore, if your protein is not too small, and you only want to remove low molecular weight substances, you can use the Antibody Concentration and Clean Up Kit.
  2. I am looking to purchase NB110-8392, but need a formulation without Sodium Azide. Would a product like this be available?
    • I have searched our product database for an IL3 antibody without sodium azide but was not successful. My recommendation would be to go ahead and purchase the antibody you were originally looking at, NB110-8392, and using our AbSelect Antibody Purification Kit, cat#860-0005 to clean it up. This kit is designed for removing substances such as azide, BSA, glycine and tris.

Additional AbSelect Products

AbSelect 860-0005

Blogs on AbSelect.

Easy Antibody Labeling, Regardless of Buffer or Concentration!
Novus Biologicals is excited to announce the release of a new line of Antibody Purification Kits, for use with our popular Lightning Link Antibody Labeling Kits. These new kits allow researchers to label any antibody, regardless of the buffer or conce...  Read full blog post.

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Alissa Taylor
09/24/2014
Application: ELISA
Species: Human