Abeta 40 Antibody

Western Blot: Abeta 40 Antibody [NB300-225] - Detection of Abeta 40 on 5 ng of peptide per lane using NB300-225. Lane 1: Abeta 40, lane 2: Abeta 42, Lane 3: Abeta 40 and 42 mix.

Immunocytochemistry/Immunofluorescence: Abeta 40 Antibody [NB300-225] - The Abeta 40 antibody was tested in SH-SY5Y cells at a 1:1000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Product Discontinued

Abeta 40 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acid residues GLMVGGVV of human Abeta [UniProt# P05067]
• Specificity: Specific for Abeta 40; there is no cross-reactivity with Abeta 42.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APP
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:1000
• Application Notes: This Abeta 40 antibody is useful for Western blot, Immunoprecipitation and Immunocytochemistry/Immunofluorescence.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Abeta 40 Antibody

• amyloid beta 40
• Beta-amyloid protein 40

Background

Alzheimer's disease (AD) is a devastating neurodegenerative ailment which affects mainly the elderly people and leaves them with progressive intellectual deterioration involving memory, language, judgment, problem solving capability etc. and it is characterized by the accumulation of senile plaques (in brain tissue) composed of amyloid-beta (Abeta) peptides predominantly consisting of 40 (Abeta 40) and 42 (Abeta 42) amino acids which are generated via processing of APP (amyloid precursor protein). APP gets hydrolyzed by beta-secretase to generate a 99-residue membrane-associated C-terminus fragment (APP-C99) which is further cleaved to release a ~4-kDa peptide, Abeta, and AICD (APP intracellular domain) via an unusual form of proteolysis involving gamma-secretase mediated protein cleavage within the transmembrane domain (at residue +40 or +42). On the other hand, alpha-secretase cleaves APP in the middle of the abeta region resulting to the release of a large ectodomain (alpha-APPs), leaveing behind a C-terminus fragment of 83 amino acids (APP-C83) in the membrane which is further cleaved by gamma-secretase to generate p3, an N-terminally truncated form of abeta. The excessive accumulation of abeta peptides in AD is generally due to increased endoproteolytic cleavage of membrane bound APP, over-expression of APP and/or decreased clearance of abeta from the CNS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Abeta 40 Antibody (NB300-225)(2)

Publications using NB300-225

• Ezpeleta J, Baudouin V, Arellano-Anaya ZE et al. Production of seedable Amyloid-beta peptides in model of prion diseases upon PrPSc-induced PDK1 overactivation Nat Commun 2019-08-01 [PMID: 31371707] (IP, Mouse)
• Pradines E, Hernandez-Rapp J, Villa-Diaz A et al. Pathogenic prions deviate PrP(C) signaling in neuronal cells and impair A-beta clearance Cell Death Dis 2013-01-10 [PMID: 23303130] (IP, Mouse)

FAQs for Abeta 40 Antibody (NB300-225). (Showing 1 - 1 of 1 FAQs).

1. What is the molecular weight and molar concentration of Abeta 40 Antibody (NB300-225)?
   • IgG molecules have 2 heavy (~55 kDa) and 2 light (~25 kDa) chains, and so the size of an IgG is 55 kDa x 2 + 25 kDa x 2 = ~160 kDa.  Also all IgG molecules have the similar sizes on PAGE gels.  Regarding the concentration, because this antibody is unpurified rabbit antiserum, we don't know the concentration and apologize for any inconvenience.  We provide recommended dilutions for each validated application (Western Blot 1:500-1:1000, Immunocytochemistry/Immunofluorescence 1:1000, and Immunoprecipitation 1:100), and have a 100% guarantee for verified applications and species

Bioinformatics

• Gene Symbol: APP
• Entrez:
  • 351(Human)
• Uniprot:
  • P05067()