ABCG5 Antibody (1B5E10) - Azide and BSA Free

Western Blot: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - Western blot analysis of ABCG5 in human ABCG5 recombinant protein. (Expected MW is 32.7 kDa) Image from the standard format of this antibody.

Immunohistochemistry: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - IHC analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining. Image from the standard format of this antibody.

Flow Cytometry: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - Flow cytometry analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red). Image from the standard format of this antibody.

Western Blot: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - Western blot analysis of ABCG5 in (1) HEK293 and (2) ABCG5 hIgGFc transfected HEK293 cell lysate. Image from the standard format of this antibody.

Western Blot: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - Western blot analysis of ABCG5 in (1) HL7702, (2) RAJI and (3) Jurkat cell lysate. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: ABCG5 Antibody (1B5E10) - Azide and BSA Free [NBP2-80559] - IHC analysis of paraffin-embedded human cervical cancer cancer tissues with DAB staining. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC/IF
• Clone: 1B5E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

ABCG5 Antibody (1B5E10) - Azide and BSA Free Summary

• Immunogen: Partial recombinant human ABCG5 (between residues 300-500) expressed in E. coli [UniProt Q9H222].
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCG5
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA : 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry : 1:200 - 1:2000
  • Immunohistochemistry-Paraffin : 1:200 - 1:2000
  • Western Blot : 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for ABCG5 Antibody (1B5E10) - Azide and BSA Free

• ATP-binding cassette sub-family G member 5
• ATP-binding cassette, sub-family G (WHITE), member 5
• ATP-binding cassette, subfamily G, member 5
• sterolin 1
• sterolin-1
• STSL

Background

ABCG5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCG5 is a member of the White subfamily. It functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. ABCG5 is expressed in a tissue-specific manner in the liver, colon, and intestine. Mutations in this protein may contribute to sterol accumulation and atheroschlerosis, and they have been observed in patients with sitosterolemia (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABCG5