ABCG2/CD338 Antibody (BXP-53)



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ABCG2/CD338 Antibody (BXP-53) Summary

The antibody reacts with the ABCG2 antigen.
Stem Cell Marker
The BXP-53 Mab was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394. BXP-53 reacts with an internal epitope of bcrp, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. BXP-53 also reacts with the human BCRP molecule.
Tissue culture supernatant
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
serumfree culture supernatant, with 0.7% BSA. Concentration approx. 250 ug Ig/ml
0.1% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.
Tissue culture supernatant


Application Notes
Immunocytochemistry: use 1:20-50 dil. on acetone fixed cytospin preparations. For immunohistochemistry: BXP-53 (use 1:20) on acetone fixed frozen sections can be followed by incubation with biotin-labeled rabbit anti-rat IgG (1:100) and HRP-labeled


The 5D3 monoclonal antibody reacts with the extracellular portion of the human ABCG2 protein, also known as Bcrp1 and MXR. The ABCG2 gene, a member of the multi-drug resistance (MDR) family, is highly expressed on primitive side-population (SP) stem cells, which are defined by the efflux of fluorescent dyes such as Rhodamine 123 and Hoechest 33342. In the bone marrow, about 0.05% of cells display the low fluorescence and are highly enriched for repopulating cells. The SP cells, which express low or undetectable levels of CD34, have been identified in multiple species. In addition, expression of ABCG2 appears to be highly conserved.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol ABCG2

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