A-RAF Antibody

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Western Blot: ARAF1 Antibody [NBP1-71858] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-A-RAF antibody used for IP at 6 mcg/mg lysate. A-RAF was also ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-71858
    • Availability
      Product Discontinued

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A-RAF Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1 and 50 of human A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase using the numbering given in entry NP_001645.1 (GeneID 369).
Clonality
Polyclonal
Host
Rabbit
Gene
ARAF
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoprecipitation 2-10 mcg/mg

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for A-RAF Antibody

  • ARaf
  • A-Raf
  • ARAF1A-RAF
  • EC 2.7.11
  • EC 2.7.11.1
  • Oncogene ARAF1
  • PKS
  • PKS2
  • PKS2A-Raf proto-oncogene serine/threonine-protein kinase
  • Proto-oncogene A-Raf
  • Proto-oncogene A-Raf-1
  • Proto-oncogene Pks
  • RAFA1
  • Ras-binding protein DA-Raf
  • serine/threonine-protein kinase A-Raf
  • v-raf murine sarcoma 3611 viral oncogene homolog 1
  • v-raf murine sarcoma 3611 viral oncogene homolog

Background

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARAF
Entrez
Uniprot