8-Hydroxyguanosine Antibody

Images

 
Immunohistochemistry: 8-Hydroxyguanosine Antibody [NB600-1508] - Goat anti-8-OHdG Alzheimer disease brain showing immunohistochemcal staining of oxidized DNA in neurons. Tissue sections were formalin-fixed, ...read more
Immunohistochemistry: 8-Hydroxyguanosine Antibody [NB600-1508] - Goat anti-8-OHdG Alzheimer disease brain showing immunohistochemical staining of oxidized DNA in neurons. Tissue sections were formalin-fixed, ...read more

Product Details

Summary
Reactivity Hu, NASpecies Glossary
Applications ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat

Order Details

8-Hydroxyguanosine Antibody Summary

Immunogen
8-Hydroxyguanosine - conjugate.
Localization
Nuclear
Specificity
NB600-1508 cross reacts completely with 8-OHdG, and does not cross react with other naturally occurring nucleotides.
Clonality
Polyclonal
Host
Goat
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Whole antisera
Preservative
0.01% Thimerosal
Purity
Unpurified

Applications/Dilutions

Dilutions
  • ELISA 1:100000-1:250000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
Application Notes
For IHC-Paraffin: It is suggested that the tissues are pretreated with 10 ug/mL of proteinase K for 40 minutes at 37 degrees C prior to staining. Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% serum in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37 degrees C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.

Reactivity Notes

Due to the conserved nature of the target cross reactivity would be expected in wide range of species.

Alternate Names for 8-Hydroxyguanosine Antibody

  • 8 OHG
  • 8-hydroxy-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 8-Hydroxyguanosine Antibody (NB600-1508) (0)

There are no publications for 8-Hydroxyguanosine Antibody (NB600-1508).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 8-Hydroxyguanosine Antibody (NB600-1508) (0)

There are no reviews for 8-Hydroxyguanosine Antibody (NB600-1508). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for 8-Hydroxyguanosine Antibody (NB600-1508). (Showing 1 - 1 of 1 FAQ).

  1. NB600-1508 could be applied on mouse tissue?
    • Since 8-hydroxyguanosine is a modified base which occurs in DNA as a result of oxidative stress, hence considered a sensitive marker of oxidative damage in cellular DNA, its structure is pretty conserved among different species. Therefore, the cross reactivity of this antibody may be expected in wide range of species. It is worth mentioning NB600-1508 does not cross react with other naturally occurring nucleotides.

Secondary Antibodies

 

Isotype Controls

Additional 8-Hydroxyguanosine Antibody Products

8-Hydroxyguanosine NB600-1508

Blogs on 8-Hydroxyguanosine

There are no specific blogs for 8-Hydroxyguanosine, but you can read our latest blog posts.

Contact Information

Product PDFs

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB600-1508 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought