6-Histidine Epitope Tag Antibody


Western Blot: 6-Histidine Epitope Tag Antibody [NBP1-51166] - Analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:2000 (lane 1) and 1:5000 (lane 2). Each lane was loaded with 10 ug of ...read more
Western Blot: 6-Histidine Epitope Tag Antibody [NBP1-51166] - Extracts from HEK293A cells,transfected with His-tag vector.

Product Details

Reactivity Hu, AllSpecies Glossary
Applications WB, ELISA
1.0 mg/ml

Order Details

6-Histidine Epitope Tag Antibody Summary

Synthetic peptide His-tag
His-tag pAb detects over-expressed or recombinant proteins containing the 6x His epitope tag
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate buffered saline (PBS) approx. pH 7.2.
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
Western blot (WB) analyzes of His-tag pAb in His-tagged protein purified from E.coli. Use in ELISA reported in scientific literature (PMID 24300951)
Read Publications using NBP1-51166.

Alternate Names for 6-Histidine Epitope Tag Antibody

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His tag
  • HIS
  • Poly-histidine


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 6-Histidine Epitope Tag Antibody (NBP1-51166)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Additional 6-Histidine Epitope Tag Antibody Products

6-Histidine Epitope Tag NBP1-51166

Blogs on 6-Histidine Epitope Tag.

Epitope Tagging: From His to OLLAS
Epitope tagging is a procedure that inserts a short amino acid sequence into a protein within an expression vector via genetic engineering. Antibodies that recognize the tag can then be used to detect the protein when no antibody to the target protein...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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