6-Histidine Epitope Tag Antibody [HRP]


Western Blot: 6-Histidine Epitope Tag Antibody [HRP] [NBP1-42785] - Detection of1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein ...read more

Product Details

Reactivity NASpecies Glossary
Applications WB, ELISA, IHC, IHC-P
LYOPH mg/ml

Order Details

6-Histidine Epitope Tag Antibody [HRP] Summary

This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
0.01% Gentamicin Sulfate
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water (or equivalent) to desired concetration.


  • Western Blot 1:2000-1:5000
  • ELISA 1:5000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
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Reviewed Applications
Read 1 Review rated 5
NBP1-42785 in the following applications:

Read Publications using NBP1-42785.

Alternate Names for 6-Histidine Epitope Tag Antibody [HRP]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His tag
  • HIS
  • Poly-histidine


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 6-Histidine Epitope Tag Antibody (NBP1-42785)(3)

Reviews for 6-Histidine Epitope Tag Antibody (NBP1-42785) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-42785:
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Images Ratings Applications Species Date Details
reviewed by:
WB Mouse 11/21/2011


ApplicationWestern Blot
Sample TestedMouse
CommentsGood! A single band was detected as expected


Blocking DetailsBlocking Buffer: 5% skim milk in PBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: 2 hours


Detection NotesDetection Method: ECL


CommentsGood! A single band was detected as expected

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for 6-Histidine Epitope Tag Antibody (NBP1-42785). (Showing 1 - 1 of 1 FAQs).

  1. What is the time of incubation for 6-histidine epitope tag antibody (HRP)?
    • I am not sure what application you are asking about, as NBP1-42785 is guaranteed for use in both Western blot and IHC on paraffin-embedded tissues. Generally we recommend overnight at 4 degrees C for both Western and IHC. As this is an antibody to simply a His-tag, you can probably get away with incubating for an hour or two at room temperature as well.

Secondary Antibodies


Isotype Controls

Additional 6-Histidine Epitope Tag Antibody [HRP] Products

6-Histidine Epitope Tag NBP1-42785

Blogs on 6-Histidine Epitope Tag.

Epitope Tagging: From His to OLLAS
Epitope tagging is a procedure that inserts a short amino acid sequence into a protein within an expression vector via genetic engineering. Antibodies that recognize the tag can then be used to detect the protein when no antibody to the target protein...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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