6-Histidine Epitope Tag Antibody (33D10.D2) [DyLight 405]


Fluorophore-linked immunosorbent assay: 6-Histidine Epitope Tag Antibody (33D10.D2) [DyLight 405] [NBP1-97328]

Product Details

Reactivity NASpecies Glossary
Applications WB, ELISA, FLISA
LYOPH mg/ml

Order Details

Available Conjugates

6-Histidine Epitope Tag Antibody (33D10.D2) [DyLight 405] Summary

This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Protein A purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
DyLight 405
DyLight 405 (A=400, E=420)
20 mM Potassium Phosphate (pH 7.2), 150 mM NaCl, and 10 mg/ml BSA (immunoglobulin and protease free)
0.01% Sodium Azide
LYOPH mg/ml
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


  • Western Blot 1:2000-1:5000
  • ELISA 1:20000-1:1000000
  • Fluorophore-linked immunosorbent assay 1:100-1:2000
Application Notes
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Reactivity Notes

6x His tag specific


Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Blue Excitation Wavelength: 400 Emission Wavelength: 420
Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for 6-Histidine Epitope Tag Antibody (33D10.D2) [DyLight 405]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His tag
  • HIS
  • Poly-histidine


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Additional 6-Histidine Epitope Tag Products

6-Histidine Epitope Tag NBP1-97328

Blogs on 6-Histidine Epitope Tag.

Epitope Tagging: From His to OLLAS
Epitope tagging is a procedure that inserts a short amino acid sequence into a protein within an expression vector via genetic engineering. Antibodies that recognize the tag can then be used to detect the protein when no antibody to the target protein...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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