6-Histidine Epitope Tag Antibody (33D10.D2) [HRP]


Western Blot: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression ...read more
ELISA: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and ...read more
6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - Antigen: 6X HIS-tagged conjugated BSA at the N or C terminus of the 6XHis. Coating amount: 0.15ug per welll. Primary antibody (direct detection): HRP ...read more

Product Details

Reactivity NASpecies Glossary
Applications WB, ELISA, IHC, IHC-P
LYOPH mg/ml

Order Details

Available Conjugates

6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] Summary

This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Protein A purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
0.01% Gentamicin Sulfate
LYOPH mg/ml
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water to a final concentration of 1.0 mg/ml.


Application Notes
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Readout System
Read Publication using NBP1-42783.

Reactivity Notes

6x His tag specific

Alternate Names for 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His tag
  • HIS
  • Poly-histidine


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 6-Histidine Epitope Tag Antibody (NBP1-42783)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for 6-Histidine Epitope Tag Antibody (NBP1-42783). (Showing 1 - 1 of 1 FAQ).

  1. I'm planning to use the 6-Histidine Epitope Tag Antibody for immunohistochemistry (formalin fixed, paraffin embedded) and am looking for a suitable positive control. Does Novus supply or recommend any positive controls which would be suitable?
    • This antibody is to the epitope tag, so there really isn't anything we can offer as a positive control. Any sample confirmed to have the His-tag would probably be your best bet as a positive control.

Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP1-42782

Additional 6-Histidine Epitope Tag Products

6-Histidine Epitope Tag NBP1-42783

Research Areas for 6-Histidine Epitope Tag Antibody (NBP1-42783)

Find related products by research area.

Blogs on 6-Histidine Epitope Tag.

Epitope Tagging: From His to OLLAS
Epitope tagging is a procedure that inserts a short amino acid sequence into a protein within an expression vector via genetic engineering. Antibodies that recognize the tag can then be used to detect the protein when no antibody to the target protein...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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